Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.1 mM, 108% of initial activity | Lactobacillus helveticus | |
KCl | up to 300 mM, slight activitation. Inhibitory above 500 mM | Lactobacillus helveticus | |
Na+ | up to 300 mM, slight activitation. Inhibitory above 500 mM | Lactobacillus helveticus |
Application | Comment | Organism |
---|---|---|
food industry | X-prolyl dipeptidyl aminopeptidase PepX, EC 3.4.14.11, and the general aminopeptidase N, EC 3.4.11.2, exhibit a clear synergistic effect in casein hydrolysis studies. Here, the relative degree of hydrolysis is increased by approx. 132% | Lactobacillus helveticus |
Cloned (Comment) | Organism |
---|---|
expression in Esherichia coli | Lactobacillus helveticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 89% residual activity | Lactobacillus helveticus | |
Cu2+ | 1 mM, 21% residual activiy | Lactobacillus helveticus | |
Hg2+ | 0.001 mM, 3% residual activity | Lactobacillus helveticus | |
KCl | up to 300 mM, slight activitation. Inhibitory above 500 mM | Lactobacillus helveticus | |
L-Phe-L-Pro | 10 mM, 38% inhibition, mixed-ype inhibition | Lactobacillus helveticus | |
L-Tyr-L-Pro | 10 mM, 28% inhibition, mixed-type inhibition | Lactobacillus helveticus | |
Na+ | up to 300 mM, slight activitation. Inhibitory above 500 mM | Lactobacillus helveticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
Gly-L-Pro 4-nitroanilide | pH 6.5, 37°C | Lactobacillus helveticus | |
1.3 | - |
L-Ala-L-Pro 4-nitroanilide | pH 6.5, 37°C | Lactobacillus helveticus | |
1.5 | - |
L-Arg-L-Pro 4-nitroanilide | pH 6.5, 37°C | Lactobacillus helveticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
2 * 90000, SDS-PAGE | Lactobacillus helveticus |
180000 | - |
PAGE | Lactobacillus helveticus |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 10% v/v, 50% residual activity | Lactobacillus helveticus |
Ethanol | 10% v/v, 78% residual activity | Lactobacillus helveticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus helveticus | S4TJ23 | - |
- |
Lactobacillus helveticus ATCC 120460 | S4TJ23 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-L-Pro 4-nitroanilide + H2O | 6% of the activity with L-Ala-L-Pro-4-nitroanilide | Lactobacillus helveticus | Gly-L-Pro + 4-nitroaniline | - |
? | |
Gly-L-Pro 4-nitroanilide + H2O | 6% of the activity with L-Ala-L-Pro-4-nitroanilide | Lactobacillus helveticus ATCC 120460 | Gly-L-Pro + 4-nitroaniline | - |
? | |
L-Ala-L-Pro 4-nitroanilide + H2O | - |
Lactobacillus helveticus | L-Ala-L-Pro + 4-nitroaniline | - |
? | |
L-Ala-L-Pro 4-nitroanilide + H2O | - |
Lactobacillus helveticus ATCC 120460 | L-Ala-L-Pro + 4-nitroaniline | - |
? | |
L-Arg-L-Pro 4-nitroanilide + H2O | 59% of the activity with L-Ala-L-Pro-4-nitroanilide | Lactobacillus helveticus | L-Arg-L-Pro + 4-nitroaniline | - |
? | |
L-Arg-L-Pro 4-nitroanilide + H2O | 59% of the activity with L-Ala-L-Pro-4-nitroanilide | Lactobacillus helveticus ATCC 120460 | L-Arg-L-Pro + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 90000, SDS-PAGE | Lactobacillus helveticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Lactobacillus helveticus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
8% residual activity | Lactobacillus helveticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
15 days, 90% residual activity | Lactobacillus helveticus |
50 | - |
15 days, 34% residual activity | Lactobacillus helveticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
Na2HPO4/KH2PO4 buffer | Lactobacillus helveticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | - |
87% of maximum activity, Na2HPO4/KH2PO4 buffer | Lactobacillus helveticus |