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Literature summary for 3.4.14.11 extracted from

  • Lloyd, R.J.; Pritchard, G.G
    Characterization of X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis subsp. Lactis (1991), J. Gen. Microbiol., 137, 49-55.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Lactococcus lactis
EDTA no inhibition Lactococcus lactis
iodoacetate
-
Lactococcus lactis
phenylmethylsulfonyl fluoride
-
Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Lactococcus lactis
0.0092
-
Leu-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
0.0124
-
Phe-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
0.0141
-
Gly-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
0.0146
-
Lys-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
0.0457
-
Glu-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
0.107
-
Gly-Pro 4-nitroanilide
-
Lactococcus lactis
0.231
-
Lys-Ala-7-amido-4-methylcoumarin
-
Lactococcus lactis
0.385
-
Gly-Ala 4-methylcoumarin 7-amide
-
Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
gel filtration Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
ssp. lactis
-
Lactococcus lactis
-
H1
-
Lactococcus lactis H1
-
H1
-

Purification (Commentary)

Purification (Comment) Organism
-
Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-casomorphin + H2O fragment 60-66 of beta-casein or beta-casomorphin Lactococcus lactis ? main split products: Tyr-Pro, Phe-Pro and Gly-Pro-Ile ?
Glu-Pro-7-amido-4-methylcoumarin + H2O
-
Lactococcus lactis Glu-Pro + 7-amino-4-methylcoumarin
-
?
Gly-Ala-7-amido-4-methylcoumarin + H2O
-
Lactococcus lactis Gly-Ala + 7-amino-4-methylcoumarin
-
?
Gly-Pro 4-nitroanilide + H2O
-
Lactococcus lactis Gly-Pro + 4-nitroaniline
-
?
Gly-Pro-7-amido-4-methylcoumarin + H2O
-
Lactococcus lactis Gly-Pro + 7-amino-4-methylcoumarin
-
?
Gly-Pro-Gly-Gly + H2O
-
Lactococcus lactis Gly-Pro + Gly-Gly
-
?
Leu-Pro-7-amido-4-methylcoumarin + H2O
-
Lactococcus lactis Leu-Pro + 7-amino-4-methylcoumarin
-
?
Lys-Ala-7-amido-4-methylcoumarin + H2O
-
Lactococcus lactis Lys-Ala + 7-amino-4-methylcoumarin
-
?
Lys-Ala-Val-Pro-Tyr-Pro-Gln + H2O fragment 176-182 of beta-casein Lactococcus lactis ?
-
?
Lys-Pro-7-amido-4-methylcoumarin + H2O
-
Lactococcus lactis Lys-Pro + 7-amino-4-methylcoumarin
-
?
additional information no activity with bradikinins with N-terminal sequences Arg-Pro-Pro- and Lys-Pro-Pro- Lactococcus lactis ?
-
?
Phe-Pro-7-amido-4-methylcoumarin + H2O
-
Lactococcus lactis Phe-Pro + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
dimer 2 * 82000-83000, SDS-PAGE Lactococcus lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
Gly-Ala-7-amido-4-methylcoumarin
-
Lactococcus lactis
17.1
-
Lys-Ala-7-amido-4-methylcoumarin
-
Lactococcus lactis
43.4
-
Leu-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
46.4
-
Gly-Pro-Gly-Gly
-
Lactococcus lactis
61.6
-
Lys-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
62.1
-
Gly-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
62.9
-
Glu-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
69.8
-
Gly-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis
78.4
-
Phe-Pro-7-amido-4-methylcoumarin
-
Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9 little variation in activity in this range Lactococcus lactis