Literature summary for 3.4.13.9 extracted from

Vyas, N.K.; Nickitenko, A.; Rastogi, V.K.; Shah, S.S.; Quiocho, F.A.
Structural insights into the dual activities of the nerve agent degrading organophosphate anhydrolase/prolidase (2010), Biochemistry, 49, 547-559.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified enzyme, hanging drop method, mixing of 0.002 ml of protein solution containing 10 mg/ml protein in 1 mM 2-mercaptoethanol, 0.1 mM MnCl2, and 10 mM Tris-HCl, pH 7.2, with 0.002 ml of reservoir solution containing 16% PEG 4000, 20 mM MnCl2, 1 mM 2-mercaptoethanol, 270 mM ammonium acetate, and 60 mM sodium acetate, pH 4.6, at 20°C, 2-4 days, X-ray diffraction structure determination and analysis	Alteromonas sp.

Crystallization (Comment)

Organism

purified enzyme, hanging drop method, mixing of 0.002 ml of protein solution containing 10 mg/ml protein in 1 mM 2-mercaptoethanol, 0.1 mM MnCl2, and 10 mM Tris-HCl, pH 7.2, with 0.002 ml of reservoir solution containing 16% PEG 4000, 20 mM MnCl2, 1 mM 2-mercaptoethanol, 270 mM ammonium acetate, and 60 mM sodium acetate, pH 4.6, at 20°C, 2-4 days, X-ray diffraction structure determination and analysis

Alteromonas sp.

Inhibitors

Inhibitors	Comment	Organism	Structure
N,N'-diisopropyldiamidofluorophosphate	i.e. DDFP or mipafox	Alteromonas sp.

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	the enzyme harbors binuclear Mn2+ ions within the active site	Alteromonas sp.

Organism

Organism	UniProt	Comment	Textmining
Alteromonas sp.	-	-	-
Alteromonas sp. JD6.5	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the bifunctional enzyme is active on the nerve agent organophosphate substrate diisopropyl fluorophosphate, DFP, producing N,N'-diisopropyldiamidophosphate, binding structure modelling, overview	Alteromonas sp.	?	-	?
additional information	the bifunctional enzyme is active on the nerve agent organophosphate substrate diisopropyl fluorophosphate, DFP, producing N,N'-diisopropyldiamidophosphate, binding structure modelling, overview	Alteromonas sp. JD6.5	?	-	?

Subunits

Subunits	Comment	Organism
More	The OPAA structure is composed of two domains, amino and carboxy domains, with the latter exhibiting a pita bread architecture and harboring the active site with the binuclear Mn2+ ions	Alteromonas sp.

Synonyms

Synonyms	Comment	Organism
OPAA	-	Alteromonas sp.
organophosphate acid anhydrolase	-	Alteromonas sp.
organophosphate anhydrolase/prolidase	-	Alteromonas sp.
prolidase	-	Alteromonas sp.