Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline | significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations | Homo sapiens | |
N-[N'-(2-chloroethyl)-N'-nitrosocarbamoyl]-L-proline | significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations | Homo sapiens | |
N-[N'-(3-chloropropyl)-N'-nitrosocarbamoyl]-L-proline | significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations | Homo sapiens | |
N-[N'-(4-bromophenyl)-N'-nitrosocarbamoyl]-L-proline | significantly increases MCF-7 cells prolidase activity, when used at 0.05-0.25 mM concentrations | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
carmustine | - |
Sus scrofa | |
additional information | carmustine does not inhibit MCF-7 cells prolidase activity | Homo sapiens | |
N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline | - |
Sus scrofa | |
Z-Pro | roughly 80% inhibition at 2.53 mM | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
cytosol | - |
Sus scrofa | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activation of prolidase requires preincubation with 2 mM Mn2+ | Homo sapiens | |
Mn2+ | activation of prolidase requires preincubation with 2 mM Mn2+ | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
SDS-PAGE | Homo sapiens |
56000 | - |
SDS-PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
kidney | - |
Sus scrofa | - |
MCF-7 cell | - |
Homo sapiens | - |
MDA-MB-231 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-L-Pro + H2O | - |
Homo sapiens | Gly + L-Pro | - |
? | |
Gly-L-Pro + H2O | - |
Sus scrofa | Gly + L-Pro | - |
? | |
glycyl-L-hydroxyproline + H2O | - |
Homo sapiens | Gly + L-hydroxyproline | - |
? | |
glycyl-L-hydroxyproline + H2O | - |
Sus scrofa | Gly + L-hydroxyproline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
prolidase | - |
Homo sapiens |
prolidase | - |
Sus scrofa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.038 | - |
in 0.05 M Tris-HCl, pH 7.8, 2 mM MnCl2, at 37°C | Sus scrofa | carmustine | |
0.082 | - |
in 0.05 M Tris-HCl, pH 7.8, 2 mM MnCl2, at 37°C | Sus scrofa | N-[N'-(2-bromoethyl)-N'-nitrosocarbamoyl]-L-proline |