Cloned (Comment) | Organism |
---|---|
expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21 (DE3) under the control of the cytomegalovirus promoter and the SP163 enhancer | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme decficiency leads to a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract, genotype/phenotype relationship, overview | Homo sapiens |
General Stability | Organism |
---|---|
Mn2+ and GSH stabilize the untagged recombinant enzyme | Homo sapiens |
PEG and glycerol stabilize the enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-Benzyloxycarbonyl-L-proline | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can substitute for Mn2+ by less than 30% | Homo sapiens | |
Co2+ | can substitute for Mn2+ by less than 30% | Homo sapiens | |
Mg2+ | can substitute for Mn2+ by less than 30% | Homo sapiens | |
Mn2+ | dependent on, best divalent cation | Homo sapiens | |
Zn2+ | can substitute for Mn2+ by less than 30% | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | prolidase deficiency causes a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity, on-column digestion of the N-terminal His-tag by factor Xa | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
hydrolysis of Xaa-/-Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro-Pro | catalytic mechanism | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
37°C, purified recombinant enzyme, loss of almost all activity after 2 days, 70% remaining activity after one day for the detagged enzyme, 47% remaining activity for the tagged enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro + H2O | - |
Homo sapiens | Ala + Pro | - |
? | |
Gly-Pro + H2O | - |
Homo sapiens | Gly + Pro | - |
? | |
Leu-Pro + H2O | - |
Homo sapiens | Leu + Pro | - |
? | |
additional information | prolidase deficiency causes a rare autosomal recessive disease, characterized by a wide range of clinical outcomes, including severe skin lesions, mental retardation, and infections of the respiratory tract | Homo sapiens | ? | - |
? | |
additional information | prolidase is a Mn2+-dependent exo- or dipeptidase that cleaves imidodipeptides containing C-terminal proline or hydroxyproline, substrate specificity of native and recombinant enzymes, molecular modeling, overview | Homo sapiens | ? | - |
? | |
Phe-Pro + H2O | - |
Homo sapiens | Phe + Pro | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, recombinant enzyme, SDS-PAGE | Homo sapiens |
More | molecular modeling | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the metallopeptidase family | Homo sapiens |
prolidase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | 50% of maximal activity at 37°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Homo sapiens |