Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.13.23 extracted from

  • Emter, R.; Natsch, A.
    The sequential action of a dipeptidase and a beta-lyase is required for the release of the human body odorant 3-methyl-3-sulfanylhexan-1-ol from a secreted Cys-Gly-S conjugate by Corynebacteria (2008), J. Biol. Chem., 283, 20645-20652 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.13.23

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Corynebacterium striatum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Corynebacterium striatum
pyridine-2,6-dicarboxylic acid
-
 Corynebacterium striatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 S-(1-hydroxy-3-methylhexan-3-yl)-L-cysteinylglycine pH 7.0, temperature not specified in the publication Corynebacterium striatum
0.2
-
 S-benzyl-L-Cys-Gly pH 7.0, temperature not specified in the publication Corynebacterium striatum

Organism

Organism UniProt Comment Textmining
Corynebacterium striatum B2KZE7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Glu-L-Trp + H2O
-
 Corynebacterium striatum L-glutamate + L-tryptophan
-
 ?
L-Ile-L-Asn + H2O
-
 Corynebacterium striatum L-isoleucine + L-asparagine
-
 ?
L-Leu-L-Ala + H2O
-
 Corynebacterium striatum L-leucine + L-alanine
-
 ?
L-Leu-L-Asn + H2O
-
 Corynebacterium striatum L-leucine + L-asparagine
-
 ?
L-Trp-L-Leu + H2O
-
 Corynebacterium striatum L-tryptophan + L-leucine
-
 ?
L-Val-L-Lys + H2O
-
 Corynebacterium striatum L-valine + L-lysine
-
 ?
additional information enzyme hydrolyzes a relatively broad range of dipeptides such as Trp-Leu, Glu-Trp, Ile-Asn, Leu-Ala, but it is not able to cleave acidic amino acids from the C terminus or to cleave Cys-Gly Corynebacterium striatum ?
-
 ?
S-(1-hydroxy-3-methylhexan-3-yl)-L-cysteinylglycine + H2O
-
 Corynebacterium striatum S-(1-hydroxy-3-methylhexan-3-yl)-L-cysteine + glycine
-
 ?
S-benzyl-L-Cys-Gly + H2O
-
 Corynebacterium striatum S-benzyl-L-Cys + glycine
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Corynebacterium striatum

Synonyms

Synonyms Comment Organism
thiol precursor dipeptidase
-
 Corynebacterium striatum
tpdA
-
 Corynebacterium striatum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.015
-
 pH 7.0, temperature not specified in the publication Corynebacterium striatum 1,10-phenanthroline
0.015
-
 pH 7.0, temperature not specified in the publication Corynebacterium striatum pyridine-2,6-dicarboxylic acid

General Information

General Information Comment Organism
physiological function enzyme is involved in formation of human axillary odor by the action of Corynebacteria on odorless axilla secretions. Co-incubation of either a synthetic Cys-Gly-S conjugate or fresh axilla secretions with both the C-S lyase and the dipeptidase TpdA releases the odorant 3-methyl-3-sulfanylhexan-1-ol Corynebacterium striatum