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Literature summary for 3.4.13.22 extracted from

  • Tan, A.L.; Loke, P.; Sim, T.S.
    Molecular cloning and functional characterisation of VanX, a D-alanyl-D-alanine dipeptidase from Streptomyces coelicolor A3(2) (2002), Res. Microbiol., 153, 27-32.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene vanX, genomic organization, functional expression of the soluble enzyme in Escherichia coli BL21(DE3) Streptomyces coelicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22700
-
x * 22700, recombinant enzyme, SDS-PAGE Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Ala-D-Ala + H2O Streptomyces coelicolor
-
D-Ala + D-Ala
-
?
D-Ala-D-Ala + H2O Streptomyces coelicolor A3(2)
-
D-Ala + D-Ala
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces coelicolor Q9XAK6 strain M130
-
Streptomyces coelicolor A3(2) Q9XAK6 strain M130
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.6
-
recombinant enzyme Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-D-Ala + H2O
-
Streptomyces coelicolor D-Ala + D-Ala
-
?
D-Ala-D-Ala + H2O enzyme is required for vancomycin resistance Streptomyces coelicolor D-Ala + D-Ala
-
?
D-Ala-D-Ala + H2O
-
Streptomyces coelicolor A3(2) D-Ala + D-Ala
-
?
D-Ala-D-Ala + H2O enzyme is required for vancomycin resistance Streptomyces coelicolor A3(2) D-Ala + D-Ala
-
?

Subunits

Subunits Comment Organism
? x * 22700, recombinant enzyme, SDS-PAGE Streptomyces coelicolor

Synonyms

Synonyms Comment Organism
D-alanyl-D-alanine dipeptidase
-
Streptomyces coelicolor
M15.011 Merops-ID Streptomyces coelicolor
VanX
-
Streptomyces coelicolor