Literature summary for 3.4.13.22 extracted from

Araoz, R.; Anhalt, E.; Rene, L.; Badet-Denisot, M.A.; Courvalin, P.; Badet, B.
Mechanism-based inactivation of VanX, a D-alanyl-D-alanine dipeptidase necessary for vancomycin resistance (2000), Biochemistry, 39, 15971-15979.

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Inhibitors

Inhibitors	Comment	Organism	Structure
D-Ala-D-(2-difluorothio)glycine-OH	dipeptide-like mechanism-based inhibitor, cleavage of the substance results in formation of a highly reactive 4-thioquinone fluoromethide which covalently reacts with the enzyme resulting in irreversible inhibition, inhibition mechanism	Enterococcus sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	D-Ala-D-Ala	pH 7.0, 37°C	Enterococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-Ala-D-Ala + H2O	Enterococcus sp.	enzyme is required for vancomycin resistance in enterococci	D-Ala + D-Ala	-	?

Organism

Organism	UniProt	Comment	Textmining
Enterococcus sp.	-	purified recombinant enzyme overexpressed in Escherichia coli	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-Ala-D-Ala + H2O	-	Enterococcus sp.	D-Ala + D-Ala	-	?
D-Ala-D-Ala + H2O	enzyme is required for vancomycin resistance in enterococci	Enterococcus sp.	D-Ala + D-Ala	-	?

Synonyms

Synonyms	Comment	Organism
D-Ala-D-Ala amino dipeptidase	-	Enterococcus sp.
M15.011	Merops-ID	Enterococcus sp.
VanX	-	Enterococcus sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Enterococcus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
41	-	D-Ala-D-Ala	pH 7.0, 37°C	Enterococcus sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Enterococcus sp.

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.03	-	D-Ala-D-(2-difluorothio)glycine-OH	pH 7.0, 37°C	Enterococcus sp.

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Release 2023.1 (January 2023)