Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Ala-D-(2-difluorothio)glycine-OH | dipeptide-like mechanism-based inhibitor, cleavage of the substance results in formation of a highly reactive 4-thioquinone fluoromethide which covalently reacts with the enzyme resulting in irreversible inhibition, inhibition mechanism | Enterococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
D-Ala-D-Ala | pH 7.0, 37°C | Enterococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala-D-Ala + H2O | Enterococcus sp. | enzyme is required for vancomycin resistance in enterococci | D-Ala + D-Ala | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus sp. | - |
purified recombinant enzyme overexpressed in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala-D-Ala + H2O | - |
Enterococcus sp. | D-Ala + D-Ala | - |
? | |
D-Ala-D-Ala + H2O | enzyme is required for vancomycin resistance in enterococci | Enterococcus sp. | D-Ala + D-Ala | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-Ala-D-Ala amino dipeptidase | - |
Enterococcus sp. |
M15.011 | Merops-ID | Enterococcus sp. |
VanX | - |
Enterococcus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Enterococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
41 | - |
D-Ala-D-Ala | pH 7.0, 37°C | Enterococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Enterococcus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
D-Ala-D-(2-difluorothio)glycine-OH | pH 7.0, 37°C | Enterococcus sp. |