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Literature summary for 3.4.13.22 extracted from
- Phosphinate analogs of D-, D-dipeptides: slow-binding inhibition and proteolysis protection of VanX, a D-, D-dipeptidase required for vancomycin resistance in Enterococcus faecium (1995), Proc. Natl. Acad. Sci. USA, 92, 11603-11607.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Enterococcus faecium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 96% inhibition at 1 mM | Enterococcus faecium |  |
| D-3-[(1-aminoethyl)phosphinyl]-D-2-methylpropionic acid | phosphinate analogue of the proposed tetrahedral intermediate of the hydrolysis reaction, slow binding | Enterococcus faecium | |
| D-Ala-L-Ala | - |
Enterococcus faecium | |
| L-Ala-D-Ala | - |
Enterococcus faecium | |
| L-Ala-L-Ala | - |
Enterococcus faecium | |
| Zn2+ | 72% inhibition at 1 mM | Enterococcus faecium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Enterococcus faecium | |
| 1.37 | - |
D-Ala-D-Ala | pH 8.0, 37°C | Enterococcus faecium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1.2fold activation at 1 mM | Enterococcus faecium | |
| Ni2+ | 1.3fold activation at 1 mM | Enterococcus faecium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala-D-Ala + H2O | Enterococcus faecium | enzyme is essential for vancomycin resistance in Enterococcus faecium | D-Ala + D-Ala | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecium | - |
- |
- |
| Enterococcus faecium BM4147 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from Escherichia coli, to homogeneity | Enterococcus faecium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala-D-Ala + H2O | - |
Enterococcus faecium | D-Ala + D-Ala | - |
? | |
| D-Ala-D-Ala + H2O | enzyme is essential for vancomycin resistance in Enterococcus faecium | Enterococcus faecium | D-Ala + D-Ala | - |
? | |
| D-Ala-D-Ala + H2O | - |
Enterococcus faecium BM4147 | D-Ala + D-Ala | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-Ala-D-Ala-dipeptidase | - |
Enterococcus faecium |
| M15.011 | Merops-ID | Enterococcus faecium |
| VanX | - |
Enterococcus faecium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Enterococcus faecium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60 | - |
D-Ala-D-Ala | pH 8.0, 37°C | Enterococcus faecium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Enterococcus faecium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics, Ki is 0.0015 mM immediately after addition of the enzyme, but is then lowered to by a relatively slow isomerization step to a second complex to Ki = 0.00047 mM | Enterococcus faecium | |
| 58 | - |
D-Ala-L-Ala | pH 8.0, 37°C | Enterococcus faecium | |
| 98 | - |
L-Ala-L-Ala | pH 8.0, 37°C | Enterococcus faecium | |
| 225 | - |
L-Ala-D-Ala | pH 8.0, 37°C | Enterococcus faecium | |
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