Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Brucella anthropi |
Crystallization (Comment) | Organism |
---|---|
active-site modeling of DmpA. Based on the crystal structure and the catalytic mechanism proposed for DmpA, carnosine is manually introduced into the active site of the enzyme. The model shows that carnosine fits excellently, in line with the high specific activity for this substrate | Brucella anthropi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
beta-Ala-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
0.019 | - |
beta-homoAla-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
1.1 | - |
beta-homoPhe-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brucella anthropi | Q59632 | - |
- |
Brucella anthropi LMG7991 | Q59632 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Brucella anthropi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O | DmpA cleaves the N-terminal beta-homoAla, no further hydrolysis is observed within 13 days | Brucella anthropi | beta-homoAla + beta-homoLys-beta-homoPhe-beta-homoAla-beta-homoLys-beta-homoPhe | - |
? | |
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O | DmpA cleaves the N-terminal beta-homoAla, no further hydrolysis is observed within 13 days | Brucella anthropi LMG7991 | beta-homoAla + beta-homoLys-beta-homoPhe-beta-homoAla-beta-homoLys-beta-homoPhe | - |
? | |
beta-Ala-4-nitroanilide + H2O | - |
Brucella anthropi | beta-Ala + 4-nitroaniline | - |
? | |
beta-Ala-4-nitroanilide + H2O | - |
Brucella anthropi LMG7991 | beta-Ala + 4-nitroaniline | - |
? | |
beta-Ala-Ile-beta-homoTyr + H2O | hydrolysis at 1.3% compared to hydrolysis of carnosine | Brucella anthropi | beta-Ala + Ile-beta-homoTyr | - |
? | |
beta-Ala-Ile-beta-homoTyr + H2O | hydrolysis at 1.3% compared to hydrolysis of carnosine | Brucella anthropi LMG7991 | beta-Ala + Ile-beta-homoTyr | - |
? | |
beta-Ala-L-His + H2O | i.e. carnosine, preferred substrate | Brucella anthropi | beta-Ala + L-His | - |
? | |
beta-Ala-L-His + H2O | i.e. carnosine, preferred substrate | Brucella anthropi LMG7991 | beta-Ala + L-His | - |
? | |
beta-homoAla-4-nitroanilide + H2O | - |
Brucella anthropi | beta-homoAla + 4-nitroaniline | - |
? | |
beta-homoAla-beta-homoLeu + H2O | hydrolysis at 55% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoAla + beta-homoLeu | - |
? | |
beta-homoLeu-Ile-beta-homoTyr + H2O | hydrolysis at 0.01% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoLeu + Ile-beta-homoTyr | - |
? | |
beta-homoPhe-4-nitroanilide + H2O | - |
Brucella anthropi | beta-homoPhe + 4-nitroaniline | - |
? | |
beta-homoSer-Ile-beta-homoTyr + H2O | hydrolysis at 0.06% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoSer + Ile-beta-homoTyr | - |
? | |
beta-homoVal-beta-homoAla-beta-homoLeu + H2O | hydrolysis at 0.19% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoVal + beta-homoAla-beta-homoLeu | - |
? | |
beta-homoVal-Ile-beta-homoTyr + H2O | hydrolysis at 0.01% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoVal + Ile-beta-homoTyr | - |
? | |
beta-homoVal-Ile-Tyr + H2O | hydrolysis at 0.09% compared to hydrolysis of carnosine | Brucella anthropi | beta-homoVal + Ile-Tyr | - |
? | |
additional information | cleaves beta- and mixed alpha,beta-peptides and amides, but a short side chain of the N-terminal beta-amino acid residue seems to be a prerequisite, since only peptides carrying N-terminal betahGly and betahAla are hydrolyzed with good efficiencies. Tripeptides carrying two consecutive N-terminal beta-homoamino acids as well as peptides with alpha-amino acids in the N-terminal position do not serve as substrates for DmpA | Brucella anthropi | ? | - |
? | |
additional information | cleaves beta- and mixed alpha,beta-peptides and amides, but a short side chain of the N-terminal beta-amino acid residue seems to be a prerequisite, since only peptides carrying N-terminal betahGly and betahAla are hydrolyzed with good efficiencies. Tripeptides carrying two consecutive N-terminal beta-homoamino acids as well as peptides with alpha-amino acids in the N-terminal position do not serve as substrates for DmpA | Brucella anthropi LMG7991 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DmpA | - |
Brucella anthropi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Brucella anthropi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
beta-homoPhe-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
30.5 | - |
beta-Ala-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
51.4 | - |
beta-homoAla-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Brucella anthropi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.027 | - |
beta-homoPhe-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
2600 | - |
beta-homoAla-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi | |
7800 | - |
beta-Ala-4-nitroanilide | pH 8.0, 25°C | Brucella anthropi |