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Literature summary for 3.4.13.20 extracted from

  • Fanuel, L.; Goffin, C.; Cheggour, A.; Devreese, B.; Van Driessche, G.; Joris, B.; Van, Beeumen, J.; Frère, J.M.
    The DmpA aminopeptidase from Ochrobactrum anthropi LMG7991 is the prototype of a new terminal nucleophile hydrolase family (1999), Biochem. J., 341, 147-155.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.13.20

Protein Variants

Protein Variants Comment Organism
G249A the precursor is not processeed at the Gly249-Ser250 peptide bond. Production of an uncleaved and inactive protein Brucella anthropi
G249D the precursor is not processeed at the Gly249-Ser250 peptide bond. Production of an uncleaved and inactive protein Brucella anthropi
S250A the precursor is not processeed at the Gly249-Ser250 peptide bond. Production of an uncleaved and inactive protein Brucella anthropi
S250C the precursor is not processeed at the Gly249-Ser250 peptide bond. Production of an uncleaved and inactive protein Brucella anthropi
S250T the precursor is not processeed at the Gly249-Ser250 peptide bond. Production of an uncleaved and inactive protein Brucella anthropi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 L-Ala-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.4
-
 L-Lys-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.4
-
 L-Arg-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.52
-
 D-Ala-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.54
-
 D-Ala-4-nitroanilide 100 mM Tris/HCl, pH 8.0, 30°C Brucella anthropi
0.6
-
 L-Ala-4-nitroanilide 100 mM Tris/HCl, pH 8.0, 30°C Brucella anthropi
3
-
 Gly-4-nitroanilide 100 mM Tris/HCl or 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13737
-
 x * 13737 + x * 26564, electrospray mass spectrometry Brucella anthropi
15000
-
 x * 15000 + x * 30000, SDS-PAGE Brucella anthropi
26564
-
 x * 13737 + x * 26564, electrospray mass spectrometry Brucella anthropi
30000
-
 x * 15000 + x * 30000, SDS-PAGE Brucella anthropi

Organism

Organism UniProt Comment Textmining
Brucella anthropi Q59632
-
-
Brucella anthropi LMG7991 Q59632
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the active protein is derived from the precursor by elimination of the N-terminal Met residue and cleavage of the Gly249-Ser250 peptide bond without additional loss of residues. The active enzyme contains two polypeptides corresponding to residues 2-249 (alpha-subunit) and 250-375 (beta-subunit) of the precursor Brucella anthropi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Ala-4-nitroanilide + H2O
-
 Brucella anthropi D-Ala + 4-nitroaniline
-
 ?
Gly-4-nitroanilide + H2O
-
 Brucella anthropi Gly + 4-nitroaniline
-
 ?
Gly-4-nitroanilide + H2O
-
 Brucella anthropi LMG7991 Gly + 4-nitroaniline
-
 ?
L-Ala-4-nitroanilide + H2O
-
 Brucella anthropi L-Ala + 4-nitroaniline
-
 ?
L-Ala-4-nitroanilide + H2O
-
 Brucella anthropi LMG7991 L-Ala + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O
-
 Brucella anthropi L-Arg + 4-nitroaniline
-
 ?
L-Arg-4-nitroanilide + H2O
-
 Brucella anthropi LMG7991 L-Arg + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O
-
 Brucella anthropi L-Lys + 4-nitroaniline
-
 ?
L-Lys-4-nitroanilide + H2O
-
 Brucella anthropi LMG7991 L-Lys + 4-nitroaniline
-
 ?
L-Phe-4-nitroanilide + H2O
-
 Brucella anthropi L-Phe + 4-nitroaniline
-
 ?
additional information the enzyme also shows activity with various dipeptides and tripeptides Brucella anthropi ?
-
 ?
additional information the enzyme also shows activity with various dipeptides and tripeptides Brucella anthropi LMG7991 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 13737 + x * 26564, electrospray mass spectrometry Brucella anthropi
? x * 15000 + x * 30000, SDS-PAGE Brucella anthropi

Synonyms

Synonyms Comment Organism
DmpA
-
 Brucella anthropi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Brucella anthropi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.11
-
 L-Lys-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.14
-
 L-Arg-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.5
-
 L-Ala-4-nitroanilide 100 mM Tris/HCl, pH 8.0, 30°C Brucella anthropi
0.56
-
 L-Ala-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
3.3
-
 D-Ala-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
4
-
 D-Ala-4-nitroanilide 100 mM Tris/HCl, pH 8.0, 30°C Brucella anthropi
70
-
 Gly-4-nitroanilide 100 mM Tris/HCl or 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Brucella anthropi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.017
-
 L-Phe-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.275
-
 L-Lys-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.35
-
 L-Arg-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
0.8
-
 L-Ala-4-nitroanilide 100 mM Tris/HCl, pH 8.0, 30°C Brucella anthropi
1.55
-
 L-Ala-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
6.3
-
 D-Ala-4-nitroanilide 50 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi
7.5
-
 D-Ala-4-nitroanilide 100 mM Tris/HCl, pH 8.0, 30°C Brucella anthropi
23
-
 Gly-4-nitroanilide 100 mM Tris/HCl or 0 mM potassium phosphate, pH 8.0, 30°C Brucella anthropi