Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain BL21(DE3)pLysS | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
complexed with bestatin together with Zn2+, hanging drop vapour diffusion method, using 20% polyethylene glycol 3350 and 0.2 M potassium fluoride | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
H228A | inactive | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bestatin | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | the enzymatic activity of carnosinase CN2 requires Mn2+ | Mus musculus | |
additional information | Zn2+ is not required for enzymatic activity | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose bead chromatography and Superdex 200 gel filtration | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-Ala-L-His + H2O | i.e. carnosine | Mus musculus | beta-Ala + L-His | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
carnosinase | - |
Mus musculus |
CN2 | - |
Mus musculus |