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Literature summary for 3.4.13.20 extracted from

  • Teufel, M.; Saudek, V.; Ledig, J.P.; Bernhardt, A.; Boularand, S.; Carreau, A.; Cairns, N.J.; Carter, C.; Cowley, D.J.; Duverger, D.; Ganzhorn, A.J.; Guenet, C.; Heintzelmann, B.; Laucher, V.; Sauvage, C.; Smirnova, T.
    Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase (2002), J. Biol. Chem., 278, 6521-31.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
D166A mutation in putative metal binding site, complete loss of activity Homo sapiens
E201A mutation in putative metal binding site, complete loss of activity Homo sapiens
E201A/D229A mutation in putative metal binding site, complete loss of activity Homo sapiens
H133A mutation in putative metal binding site, complete loss of activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
bestatin
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
homocarnosine pH 7.5, 30°C Homo sapiens
1.27
-
beta-Ala-L-His pH 7.5, 30°C Homo sapiens
1.9
-
homocarnosine presence of CD2+, pH 7.5, 30°C Homo sapiens
11
-
beta-Ala-L-His presence of CD2+, pH 7.5, 30°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Homo sapiens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ increase in kcat value Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52800
-
2 * 82000, SDS-PAGE, 2 * 63700, MALDI-TOF, 2 * 52800, calculated Homo sapiens
63700
-
2 * 82000, SDS-PAGE, 2 * 63700, MALDI-TOF, 2 * 52800, calculated Homo sapiens
82000
-
2 * 82000, SDS-PAGE, 2 * 63700, MALDI-TOF, 2 * 52800, calculated Homo sapiens
167000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q96KN2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein after treatment with PNGase F, molecular mass shifts from 82000 Da to 62000 Da, SDS-PAGE Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain of adult. No expression in fetal brain Homo sapiens
-
central nervous system
-
Homo sapiens
-
liver low expresion Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-Ala-L-His + H2O
-
Homo sapiens beta-Ala + L-His
-
?
Gly-L-His + H2O
-
Homo sapiens Gly + L-His
-
?
homocarnosine + H2O
-
Homo sapiens ?
-
?
L-Ala-L-His + H2O
-
Homo sapiens L-Ala + L-His
-
?
additional information non Xaa-His dipeptides like Ala-Ala, or Ala-Pro, as well as tripeptides containing histidine in central or C-terminal position such as Gly-His-Gly or Gly-Gly-His, are not degraded Homo sapiens ?
-
?
N-methylcarnosine + H2O
-
Homo sapiens N-methyl-beta-Ala + L-His
-
?

Subunits

Subunits Comment Organism
dimer 2 * 82000, SDS-PAGE, 2 * 63700, MALDI-TOF, 2 * 52800, calculated Homo sapiens

Synonyms

Synonyms Comment Organism
CN1
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
homocarnosine pH 7.5, 30°C Homo sapiens
10.6
-
beta-Ala-L-His pH 7.5, 30°C Homo sapiens
18
-
homocarnosine presence of CD2+, pH 7.5, 30°C Homo sapiens
138
-
beta-Ala-L-His presence of CD2+, pH 7.5, 30°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 broad Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000007
-
bestatin pH 7.5, 30°C Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens calculated
-
4.5