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Literature summary for 3.4.13.20 extracted from

  • Riedl, E.; Koeppel, H.; Brinkkoetter, P.; Sternik, P.; Steinbeisser, H.; Sauerhoefer, S.; Janssen, B.; van der Woude, F.J.; Yard, B.A.
    A CTG polymorphism in the CNDP1 gene determines the secretion of serum carnosinase in Cos-7 transfected cells (2007), Diabetes, 56, 2410-2413.
    View publication on PubMed

Application

Application Comment Organism
medicine CNDP1 secretion is significantly higher in cells expressing variants with more than five leucines in the signal peptide of the protein. This explains why individuals homozygous for the 5L allele have low serum carnosinase activity and why diabetic patients homozygous for CNDP1 5L are protected against diabetic nephropathy Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in COS-7 cell Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information expression of constructs varying in CTG repeats coding for 4,5,6,7,or 8 leucines in the signal peptide. Enzyme secretion is significantly higher in cells expressing variants with more than five leucins in the signal peptide Homo sapiens
additional information transfection of Cos-7 cells with different gene constructs varying in CTG repeats. CNDP1 secretion is significantly higher in cells expressing variants with more than five leucines in the signal peptide of the protein. With increasing length of the CTG repeat, the percentage of secreted carnosinase increases Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
extracellular
-
Homo sapiens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
86000
-
x* 92000, SDS-PAGE of secreted protein, x * 86000 and x * 88000, SDS-PAGE of cytosolic protein Homo sapiens
88000
-
x* 92000, SDS-PAGE of secreted protein, x * 86000 and x * 88000, SDS-PAGE of cytosolic protein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens
-
isoform CDNP1
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein secreted enzyme shows a molecular weight of 92000 Da, cytosolic protein of 86000 Da and 88000 Da, respectively. PNGase-treated protein has a molecular weight of 86000 Da Homo sapiens

Subunits

Subunits Comment Organism
? x* 92000, SDS-PAGE of secreted protein, x * 86000 and x * 88000, SDS-PAGE of cytosolic protein Homo sapiens