Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Codon Plus-RIL cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | includes a bis-zinc-containing active center, in which both zinc ions have catalytic properties, folding efficiency is not improved by including up to 25 mM ZnCl2 in the folding buffer, but concentrations above 50 mM ZnCl2 cause precipitation of the protein | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultracentrifugation and Superose 12 (10/300) gel filtration | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
in 20 mM Tris-HCl, pH 8.0, 500 mM arginine, containing 1 mM reduced glutathione, 0.2 mM oxidized glutathione, and sodium chloride | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hemoglobin + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
LDP | - |
Homo sapiens |
lysosomal dipeptidase | - |
Homo sapiens |
Ser-Met dipeptidase | original name | Homo sapiens |