Cloned (Comment) | Organism |
---|---|
expression in Cos-1 cells | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
H128L | mutant enzyme exhibits a specific activity and Km for Gly-D-Phe comparable with that of the wild-type enzyme | Sus scrofa |
H152L | inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type, the mutant enzyme fails to bind to cilastatin-Sepharose in contrast to the wild-type enzyme | Sus scrofa |
H198K | inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type | Sus scrofa |
H20L | inactive mutant enzyme is expressed at the cell surface at equivalent levels to the wild-type | Sus scrofa |
H49K | mutant enzyme exhibits a specific activity and Km for Gly-D-Phe comparable with that of the wild-type enzyme | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cilastatin | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.25 | - |
Gly-D-Phe | mutant enzyme H128L | Sus scrofa | |
7.1 | - |
Gly-D-Phe | wild-type enzyme | Sus scrofa | |
12.5 | - |
Gly-D-Phe | mutant enzyme H49K | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | plasma membrane | Sus scrofa | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene D4 + H2O | Sus scrofa | the enzyme is involved in the reanal metabolism of glutathione and its conjugates such as leukotriene | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-D-Phe + H2O | - |
Sus scrofa | Gly + D-Phe | - |
? | |
leukotriene D4 + H2O | the enzyme is involved in the reanal metabolism of glutathione and its conjugates such as leukotriene | Sus scrofa | ? | - |
? |