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Literature summary for 3.4.13.19 extracted from

  • Cho, H.Y.; Tanizawa, K.; Soda, K.
    Role of divalent metal ions on activity and stability of thermostable dipeptidase from Bacillus stearothermophilus (1997), Biosci. Biotechnol. Biochem., 61, 1688-1692.
    View publication on PubMed

General Stability

General Stability Organism
substitution of Co2+ or Mn2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
Geobacillus stearothermophilus
Ca2+
-
Geobacillus stearothermophilus
Cu2+
-
Geobacillus stearothermophilus
Hg2+
-
Geobacillus stearothermophilus
Mg2+
-
Geobacillus stearothermophilus
Zn2+ slight Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ activates Geobacillus stearothermophilus
Co2+ activates,substitution of Co2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein Geobacillus stearothermophilus
Mn2+ activates Geobacillus stearothermophilus
Mn2+ the Mn-reconstituted enzyme has nearly twice the activity of the original Zn-enzyme, substitution of Mn2+ for Zn2+ considerably loweres the thermostability of the enzyme without affecting the overall conformation of the protein Geobacillus stearothermophilus
Zinc the metalloenzyme contains 1 gatom of zinc per mol of subunit, the prosthetic Zn plays dual roles in conformational stability and catalysis of the thermostable dipeptidase Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
substitution of Co2+ or Mn2+ for Zn2+ considerably lowers the thermostability of the enzyme without affecting the overall conformation of the protein Geobacillus stearothermophilus
70
-
30 min, about 15% loss of activity of the native enzyme, about 55% of the activity of the Co2+-reconstituted enzyme, about 75% loss of activity of the Mn2+-reconstituted enzyme Geobacillus stearothermophilus