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Literature summary for 3.4.13.19 extracted from

  • Sugiura, M.; Ito, Y.; Hirano, K.; Sawaki, S.
    Purification and properties of human kidney dipeptidases (1978), Biochim. Biophys. Acta, 522, 541-550.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Homo sapiens
Co2+
-
Homo sapiens
cysteine
-
Homo sapiens
EDTA
-
Homo sapiens
N-bromosuccinimide
-
Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.056
-
L-Leu-L-Leu enzyme form F Homo sapiens
0.086
-
L-Leu-L-Leu enzyme form S Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 1 mM, activates Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
2 * 66000, enzyme form F, SDS-PAGE Homo sapiens
94000
-
1 * 94000 + 1 * 115000, enzyme form S, SDS-PAGE Homo sapiens
115000
-
1 * 94000 + 1 * 115000, enzyme form S, SDS-PAGE Homo sapiens
135000
-
enzyme form F, gel filtration Homo sapiens
200000
-
enzyme form S, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
2 forms different in electrophoretic mobility: form S (slow) and F (fast) Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala + H2O
-
Homo sapiens Ala + Ala
-
?
Gly-Gly + H2O
-
Homo sapiens Gly + Gly
-
?
Gly-Leu + H2O
-
Homo sapiens Gly + Leu
-
?
L-Leu-L-Leu + H2O
-
Homo sapiens L-Leu + L-Leu
-
?
Leu-Gly + H2O
-
Homo sapiens Leu + Gly
-
?
Met-Met + H2O
-
Homo sapiens Met + Met
-
?
Phe-Tyr + H2O
-
Homo sapiens Phe + Tyr
-
?

Subunits

Subunits Comment Organism
dimer 1 * 94000 + 1 * 115000, enzyme form S, SDS-PAGE Homo sapiens
dimer 2 * 66000, enzyme form F, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
enzyme form S Homo sapiens
70
-
enzyme form F Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
enzyme form S is unstable above Homo sapiens
60
-
enzyme form F is stable up to Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
enzyme form F Homo sapiens
9 11 enzyme form S Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
unstable below, both enzyme forms Homo sapiens
8
-
both enzyme forms stable Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens
-
4.8 4.7