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Literature summary for 3.4.13.18 extracted from

  • Lee, H.J.; Chen, Y.S.; Chou, C.Y.; Chien, C.H.; Lin, C.H.; Chang, G.G.; Chen, X.
    Investigation of the dimer interface and substrate specificity of prolyl dipeptidase DPP8 (2006), J. Biol. Chem., 281, 38653-38662.
    View publication on PubMed
Search for more literature for 3.4.13.18

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 insect cells and 293-T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
F822A decreased activity Homo sapiens
H859A decreased activity Homo sapiens
V833A decreased activity Homo sapiens
Y844A decreased activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 Lys-Pro-p-nitroanilide wild type enzyme Homo sapiens
0.023
-
 Leu-Pro-p-nitroanilide wild type enzyme Homo sapiens
0.027
-
 Arg-Pro-p-nitroanilide wild type enzyme Homo sapiens
0.029
-
 Met-Pro-p-nitroanilide wild type enzyme Homo sapiens
0.127
-
 Ala-Pro-p-nitroanilide wild type enzyme Homo sapiens
0.238
-
 Ser-Pro-p-nitroanilide wild type enzyme Homo sapiens
0.27
-
 Arg-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
0.325
-
 Met-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
0.348
-
 Leu-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
0.39
-
 Leu-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
0.507
-
 Lys-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
0.528
-
 Gly-Pro-p-nitroanilide wild type enzyme Homo sapiens
0.988
-
 Met-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
1.358
-
 Lys-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
1.495
-
 Ala-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
1.673
-
 Arg-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
2.219
-
 Ala-Pro-p-nitroanilide mutant enzyme V833A Homo sapiens
2.249
-
 Ser-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
2.397
-
 Ala-Pro-p-nitroanilide mutant enzyme Y844A Homo sapiens
2.668
-
 Asp-Pro-p-nitroanilide wild type enzyme Homo sapiens
2.72
-
 Ala-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
2.731
-
 Gly-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
4.599
-
 Gly-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
7.462
-
 Ser-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 2 * 105000, His-tagged enzyme, SDS-PAGE Homo sapiens
210000
-
 His-tagged enzyme, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Q-Sepharose column chromatography, and Superdex 200 HR gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Pro-p-nitroanilide + H2O
-
 Homo sapiens Ala-Pro + p-nitroaniline
-
 ?
Arg-Pro-p-nitroanilide + H2O
-
 Homo sapiens Arg-Pro + p-nitroaniline
-
 ?
Asp-Pro-p-nitroanilide + H2O
-
 Homo sapiens Asp-Pro + p-nitroaniline
-
 ?
Gly-Pro-p-nitroanilide + H2O
-
 Homo sapiens Gly-Pro + p-nitroaniline
-
 ?
Leu-Pro-p-nitroanilide + H2O
-
 Homo sapiens Leu-Pro + p-nitroaniline
-
 ?
Lys-Pro-p-nitroanilide + H2O
-
 Homo sapiens Lys-Pro + p-nitroaniline
-
 ?
Met-Pro-p-nitroanilide + H2O
-
 Homo sapiens Met-Pro + p-nitroaniline
-
 ?
Ser-Pro-p-nitroanilide + H2O
-
 Homo sapiens Ser-Pro + p-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 105000, His-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
DPP8
-
 Homo sapiens
prolyl dipeptidase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 Ser-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
0.1
-
 Leu-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
0.2
-
 Gly-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
0.2
-
 Ala-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
0.2
-
 Lys-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
0.2
-
 Ala-Pro-p-nitroanilide mutant enzyme Y844A Homo sapiens
0.3
-
 Met-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
0.4
-
 Arg-Pro-p-nitroanilide mutant enzyme F822A Homo sapiens
0.9
-
 Asp-Pro-p-nitroanilide wild type enzyme Homo sapiens
1
-
 Ala-Pro-p-nitroanilide mutant enzyme V833A Homo sapiens
1.2
-
 Arg-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
1.4
-
 Gly-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
2.4
-
 Lys-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3
-
 Met-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
3.2
-
 Lys-Pro-p-nitroanilide wild type enzyme Homo sapiens
3.8
-
 Leu-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
7
-
 Leu-Pro-p-nitroanilide wild type enzyme Homo sapiens
8
-
 Ala-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
13
-
 Ser-Pro-p-nitroanilide mutant enzyme H859A Homo sapiens
14
-
 Arg-Pro-p-nitroanilide wild type enzyme Homo sapiens
15
-
 Met-Pro-p-nitroanilide wild type enzyme Homo sapiens
19
-
 Ser-Pro-p-nitroanilide wild type enzyme Homo sapiens
22
-
 Ala-Pro-p-nitroanilide wild type enzyme Homo sapiens
25
-
 Gly-Pro-p-nitroanilide wild type enzyme Homo sapiens