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Literature summary for 3.4.13.18 extracted from
- Properties of a highly purified dipeptidase (EC 3.4.13.?) from brewers yeast (1974), Hoppe-Seyler's Z. Physiol. Chem., 355, 675-686.
General Stability
| General Stability | Organism |
|---|---|
| dialysis against distilled water causes high degree of inactivation | Saccharomyces cerevisiae |
| dilute solutions, 0.1 mg/ml in Tris buffer, pH 8.1, of the pure enzyme lose 20% of their activity on standing for one week at 4°C or 2 h at 40°C | Saccharomyces cerevisiae |
| freezing and thawing without addition of glycerol causes 50% loss of activity | Saccharomyces cerevisiae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | complex pattern of inhibition or activation with different dipeptide substrates | Saccharomyces cerevisiae |  |
| Mn2+ | complex pattern of inhibition or activation with different dipeptide substrates | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
4 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
| 130000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| frozen, in presence of 25% v/v glycerol or 0.2 M sucrose, stable for months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-Ile + H2O | - |
Saccharomyces cerevisiae | Ala + Ile | - |
? | |
| additional information | substrates must contain uncharged side chains and free alpha-amino and carboxyl groups | Saccharomyces cerevisiae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 35000, SDS-PAGE | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
pI: 6.3 | Saccharomyces cerevisiae |
| 7.8 | 8.3 | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.3 | about 35% of activity maximum at pH 6,5, activity maximum at pH 7.8-8.3 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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