General Stability | Organism |
---|---|
very unstable in the absence of Mn2+ or Mg2+ | Mycolicibacterium phlei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Mycolicibacterium phlei | |
EDTA | - |
Mycolicibacterium phlei | |
Fe2+ | - |
Mycolicibacterium phlei | |
Hg2+ | - |
Mycolicibacterium phlei | |
iodoacetamide | - |
Mycolicibacterium phlei | |
Pb2+ | - |
Mycolicibacterium phlei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.25 | - |
Leu-Gly | - |
Mycolicibacterium phlei | |
3.3 | - |
Ala-Gly | - |
Mycolicibacterium phlei | |
21 | - |
D-Leu-Gly | - |
Mycolicibacterium phlei |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | inhibits Ala-Gly hydrolysis, activates Leu-Gly hydrolysis | Mycolicibacterium phlei | |
Mg2+ | favors the activity towards the substrates Leu-Gly and Ala-Gly | Mycolicibacterium phlei | |
Mn2+ | favors the activity towards the substrates Leu-Gly and Ala-Gly | Mycolicibacterium phlei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Mycolicibacterium phlei |
88000 | - |
gel filtration | Mycolicibacterium phlei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium phlei | - |
- |
- |
Mycolicibacterium phlei 689 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium phlei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Leu-Gly + H2O | - |
Mycolicibacterium phlei | D-Leu + Gly | - |
? | |
D-Leu-Gly + H2O | - |
Mycolicibacterium phlei 689 | D-Leu + Gly | - |
? | |
L-Ala-Gly + H2O | - |
Mycolicibacterium phlei | L-Ala + Gly | - |
? | |
L-Ala-Gly + H2O | - |
Mycolicibacterium phlei 689 | L-Ala + Gly | - |
? | |
Leu-Gly + H2O | - |
Mycolicibacterium phlei | Leu + Gly | - |
? | |
Leu-Gly + H2O | - |
Mycolicibacterium phlei 689 | Leu + Gly | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000, SDS-PAGE | Mycolicibacterium phlei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycolicibacterium phlei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Mycolicibacterium phlei |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | most stable | Mycolicibacterium phlei |