General Stability | Organism |
---|---|
stable to 18 h dialysis, at 10°C against 0.02 M phosphate buffer, pH 7.3-8.3 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Escherichia coli | |
Co2+ | when substrates are cleaved rapidly by dipeptidase, addition of Co2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect | Escherichia coli | |
cysteine | - |
Escherichia coli | |
D-Forms of dipeptides | - |
Escherichia coli | |
DTT | - |
Escherichia coli | |
iodoacetate | - |
Escherichia coli | |
Mn2+ | when substrates are cleaved rapidly by dipeptidase, addition of Mn2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | when substrates are cleaved rapidly by dipeptidase, addition of Co2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect, activation is not accompanied by change in Km | Escherichia coli | |
Co2+ | enzyme contains two binding sites | Escherichia coli | |
Mn2+ | when substrates are cleaved rapidly by dipeptidase, addition of Mn2+ inhibits the reaction. The more slowly a substrate is hydrolyzed in the absence of metals, the greater the activating effect, activation is accompanied by an increase in Km | Escherichia coli | |
Mn2+ | two binding sites, at least one of which is distinct from a Co2+ site | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE | Escherichia coli |
100000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli B / ATCC 11303 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain B | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
760 | - |
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
-30°C, pH 7.5-8.5, 0.25 M sucrose, stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ala-Gly + H2O | - |
Escherichia coli | L-Ala + Gly | - |
? | |
L-Ala-Gly + H2O | - |
Escherichia coli B / ATCC 11303 | L-Ala + Gly | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | - |
Escherichia coli B / ATCC 11303 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
30 min: stable, 24 h: 40% loss of activity | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |