Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.13.12 extracted from

  • Brown, J.L.
    Purification and properties of dipeptidase M from Escherichia coli B (1973), J. Biol. Chem., 248, 409-416.
    View publication on PubMed

General Stability

General Stability Organism
generally unstable Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
EDTA Mn2+ may restore enzyme activity after EDTA treatment Escherichia coli
iodoacetate 100% inhibition at 10 mM, 10 mM mercaptoethanol restores activity Escherichia coli
p-chloromercuribenzoate 100% inhibition at 0.1 mM Escherichia coli
Zn2+
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
Met-Leu
-
Escherichia coli
0.13
-
Met-Val
-
Escherichia coli
0.53
-
Met-Thr
-
Escherichia coli
0.25 0.31 Met-Ala
-
Escherichia coli
0.57 0.6 Met-Ser
-
Escherichia coli
0.42 1.02 Met-Ile
-
Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ also protects against heat inactivation Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 47000, SDS-PAGE Escherichia coli
93000 95000 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Met-amino acid + H2O Escherichia coli
-
?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1100
-
-
Escherichia coli

Storage Stability

Storage Stability Organism
4°C, 10 mM imidazole buffer, pH 7.2, 2 mg/ml bovine serum albumin, 30 days, 50% loss of activity Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Leu-Ala + H2O
-
Escherichia coli Leu + Ala
-
ir
Ser-Ala + H2O
-
Escherichia coli Ser + Ala
-
ir
Gly-Gly + H2O
-
Escherichia coli Gly + Gly
-
ir
Met-Ala + H2O
-
Escherichia coli Met + Ala
-
ir
Met-Ser + H2O
-
Escherichia coli Met + Ser
-
ir
Met-Ile + H2O
-
Escherichia coli Met + Ile
-
ir
Met-Thr + H2O
-
Escherichia coli Met + Thr
-
ir
Met-Leu + H2O
-
Escherichia coli Met + Leu
-
ir
Met-Gly + H2O
-
Escherichia coli Met + Gly
-
ir
Ala-Met + H2O
-
Escherichia coli Ala + Met
-
ir
Thr-Ala + H2O
-
Escherichia coli Thr + Ala
-
ir
Met-Val + H2O
-
Escherichia coli Met + Val
-
?
Met-amino acid + H2O
-
Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
below, 30 min, Mn2+ protects against heat inactivation Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 9 substrate Met-Ala Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 substrate Met-Ala Escherichia coli