Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.13.12 extracted from

  • Johnson, G.L.; Brown, J.L.
    Partial purification and characterization of two peptidases from Neurospora crassa (1974), Biochim. Biophys. Acta, 370, 530-540.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Neurospora crassa
p-chloromercuribenzoate 100% inhibition at 1 mM Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25 0.31 Met-Ala
-
Neurospora crassa
0.57 0.6 Met-Ser
-
Neurospora crassa
0.42 1.02 Met-Ile
-
Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Neurospora crassa
Zn2+ 20% of activity with manganese Neurospora crassa
Co2+ 52% of activity with manganese Neurospora crassa
Fe2+ 21% of activity with manganese Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000 114000 gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Met-amino acid + H2O Neurospora crassa
-
?
-
?

Organism

Organism UniProt Comment Textmining
Neurospora crassa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
-
Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Leu-Ala + H2O
-
Neurospora crassa Leu + Ala
-
ir
Ser-Ala + H2O
-
Neurospora crassa Ser + Ala
-
ir
Ala-Ala + H2O
-
Neurospora crassa Ala + Ala
-
ir
Met-Ala + H2O best substrate Neurospora crassa Met + Ala
-
ir
Met-Met + H2O
-
Neurospora crassa Met + Met
-
ir
Met-Ser + H2O
-
Neurospora crassa Met + Ser
-
ir
Met-Ile + H2O
-
Neurospora crassa Met + Ile
-
ir
Met-Thr + H2O
-
Neurospora crassa Met + Thr
-
ir
Met-Leu + H2O
-
Neurospora crassa Met + Leu
-
ir
Ala-Leu + H2O
-
Neurospora crassa Ala + Leu
-
ir
Ala-Met + H2O
-
Neurospora crassa Ala + Met
-
ir
norleucyl-Ala + H2O
-
Neurospora crassa norleucine + Ala
-
ir
ethionyl-Ala + H2O
-
Neurospora crassa ethionine + Ala
-
ir
Thr-Ala + H2O
-
Neurospora crassa Thr + Ala
-
ir
Met-amino acid + H2O
-
Neurospora crassa ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
substrate Met-Ala Neurospora crassa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 substrate Met-Ala Neurospora crassa