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Literature summary for 3.4.11.B3 extracted from
- Isolation and characterization of an intracellular aminopeptidase from the extreme thermophilic archaebacterium Sulfolobus solfataricus (1990), Biochim. Biophys. Acta, 1033, 148-153.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetic acid | 1%, 86% inhibition | Saccharolobus solfataricus |  |
| acetonitrile | 10%, 26% inhibition | Saccharolobus solfataricus | |
| bestatin | 1 mM, 97% inhibition | Saccharolobus solfataricus | |
| EDTA | 1 mM, complete inhibition | Saccharolobus solfataricus | |
| ethanol | 10%, complete inhibition | Saccharolobus solfataricus | |
| mercaptoethanol | 1%, 56% inhibition | Saccharolobus solfataricus | |
| additional information | no inhibition by PMSF, TLCK or soybean trypsin inhibitor | Saccharolobus solfataricus | |
| SDS | 0.1%, complete inhibition | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
L-leucyl 4-nitroanilide | pH 7.0, 70°C | Saccharolobus solfataricus | |
| 0.21 | - |
L-alanyl 4-nitroanilide | pH 7.0, 70°C | Saccharolobus solfataricus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Saccharolobus solfataricus | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 1 mM, restores enzyme activity after inhibition with EDTA | Saccharolobus solfataricus | |
| Mn2+ | 1 mM, restores enzyme activity after inhibition with EDTA | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
4 * 80000, SDS-PAGE | Saccharolobus solfataricus |
| 320000 | - |
gel filtration | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.225 | - |
pH 7.0, 70°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycyl 4-nitroanilide + H2O | 11% of the activity compared to L-Leu-4-nitroanilide | Saccharolobus solfataricus | glycine + 4-nitroaniline | - |
? | |
| L-alanyl 4-nitroanilide + H2O | 118% of the activity compared to L-Leu-4-nitroanilide | Saccharolobus solfataricus | L-alanine + 4-nitroaniline | - |
? | |
| L-arginyl 4-nitroanilide + H2O | 63% of the activity compared to L-Leu-4-nitroanilide | Saccharolobus solfataricus | L-arginine + 4-nitroaniline | - |
? | |
| L-leucyl 4-nitroanilide + H2O | - |
Saccharolobus solfataricus | L-leucine + 4-nitroaniline | - |
? | |
| L-lysyl 4-nitroanilide + H2O | 15% of the activity compared to L-Leu-4-nitroanilide | Saccharolobus solfataricus | L-lysine + 4-nitroaniline | - |
? | |
| L-phenylalanyl 4-nitroanilide + H2O | 62% of the activity compared to L-Leu-4-nitroanilide | Saccharolobus solfataricus | L-phenylalanine + 4-nitroaniline | - |
? | |
| additional information | no activity with N-acetyl-L-phenylalanine, N-acetyl-L-leucine, N-benzoyl-L-arginine | Saccharolobus solfataricus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 80000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| intracellular Sulfolobus aminopeptidase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
adition of 0.05 mM each of Co2+, Mn2+, Mg2+, Ca2+ and Zn2+ has no effect on the temperature optimum. At higher concentrations of divalent metal ions a decrease of activity occurs | Saccharolobus solfataricus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 90 | 60°C: about 60% of maximal activity, 90°C: about 35% of maximal activity | Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
15 min, activity decreases to 10% in absence of Co2+, in presence of 0.05 mM the remaining activity is 76% | Saccharolobus solfataricus |
| 85 | - |
15 min, complete loss of activity in absence of Co2+, in presence of 0.05 mM the remaining activity is 60% | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Saccharolobus solfataricus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 7.5 | pH 5.5: about 45% of maximal activity, pH 7.5: about 65% of maximal activity | Saccharolobus solfataricus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | 14 h, at least 70% residual activity | Saccharolobus solfataricus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | isoelectric focusing | - |
4.4 |
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