Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | slight activation at 0.1 mM | Aspergillus oryzae | |
bacitracin | slight activation at 0.1 mM | Aspergillus oryzae | |
bestatin | slight activation at 0.1 mM | Aspergillus oryzae |
Cloned (Comment) | Organism |
---|---|
gene ampp, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21 | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Aspergillus oryzae | |
Arg-Pro | substrate inhibition at concentrations above 3 mM | Aspergillus oryzae | |
Co2+ | strong activation at 0.01-1.0 mM, inhibitory at 10 mM | Aspergillus oryzae | |
DTT | - |
Aspergillus oryzae | |
EDTA | 10 mM EDTA inhibits enzyme activity to 0.26 times of the control level | Aspergillus oryzae | |
Fe2+ | slight activation at 0.05 mM, complete inhibition at 1 mM | Aspergillus oryzae | |
Leu-Pro | substrate inhibition at concentrations above 4 mM | Aspergillus oryzae | |
additional information | no inhibition by 4-(2-aminoethyl)benzensulfonylfluoride, tosyl lysyl chloromethyl ketone, and tosyl phenylalanyl chloromethyl ketone | Aspergillus oryzae | |
Ni2+ | - |
Aspergillus oryzae | |
Ser-Pro | substrate inhibition at concentrations above 10 mM | Aspergillus oryzae | |
Zn2+ | complete inhibition at 1 mM | Aspergillus oryzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation at 10 mM | Aspergillus oryzae | |
Co2+ | strong activation at 0.01-1.0 mM, inhibitory at 10 mM | Aspergillus oryzae | |
Fe2+ | slight activation at 0.05 mM, complete inhibition at 1 mM | Aspergillus oryzae | |
Mg2+ | activates | Aspergillus oryzae | |
Mn2+ | required, best metal ion, the enzyme activity increases 27.6 times of the control level at 1 mM Mn2+, strong activation at 0.01-50 mM | Aspergillus oryzae | |
additional information | rXpmA is a metalloprotease | Aspergillus oryzae | |
NaCl | increases the enzyme activity in the concentration range 0.5-3.0 M, suggesting that the enzyme is halophilic | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
129000 | - |
recombinant His-tagged enzyme, gel filtration | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | Q2U7S5 | - |
- |
Aspergillus oryzae ATCC 42149 | Q2U7S5 | - |
- |
Aspergillus oryzae RIB 40 | Q2U7S5 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, gel filtration, and dialysis | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
177.69 | - |
pH 8.5, 50°C, purified recombinant enzyme | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Pro + H2O | - |
Aspergillus oryzae | Ala + Pro | - |
? | |
Ala-Pro + H2O | - |
Aspergillus oryzae RIB 40 | Ala + Pro | - |
? | |
Ala-Pro + H2O | - |
Aspergillus oryzae ATCC 42149 | Ala + Pro | - |
? | |
Ala-Pro-Tyr-Ala + H2O | - |
Aspergillus oryzae | Ala + Pro-Tyr-Ala | - |
? | |
Ala-Pro-Tyr-Ala + H2O | - |
Aspergillus oryzae RIB 40 | Ala + Pro-Tyr-Ala | - |
? | |
Ala-Pro-Tyr-Ala + H2O | - |
Aspergillus oryzae ATCC 42149 | Ala + Pro-Tyr-Ala | - |
? | |
additional information | the recombinant enzyme XpmA shows hydrolysis activity toward Xaa-Pro-oligopeptides, especially the two dipeptides Ala-Pro and Phe-Pro. Peptides APRTPGGRR, RPPGFSPFR, LPFFD, and Gly-Pro-Ala show poor activity with the enzyme, while the dipeptide Gly-Pro gives no activity. rXpmA also shows no activity toward three peptides with proline at the N-terminus (Pro-Ala, Pro-Leu-Gly, and Pro-Leu-Ser-Arg-Tyr-Leu-Ser-Val-Ala-Ala-Lys-Lys) | Aspergillus oryzae | ? | - |
? | |
additional information | the recombinant enzyme XpmA shows hydrolysis activity toward Xaa-Pro-oligopeptides, especially the two dipeptides Ala-Pro and Phe-Pro. Peptides APRTPGGRR, RPPGFSPFR, LPFFD, and Gly-Pro-Ala show poor activity with the enzyme, while the dipeptide Gly-Pro gives no activity. rXpmA also shows no activity toward three peptides with proline at the N-terminus (Pro-Ala, Pro-Leu-Gly, and Pro-Leu-Ser-Arg-Tyr-Leu-Ser-Val-Ala-Ala-Lys-Lys) | Aspergillus oryzae RIB 40 | ? | - |
? | |
additional information | the recombinant enzyme XpmA shows hydrolysis activity toward Xaa-Pro-oligopeptides, especially the two dipeptides Ala-Pro and Phe-Pro. Peptides APRTPGGRR, RPPGFSPFR, LPFFD, and Gly-Pro-Ala show poor activity with the enzyme, while the dipeptide Gly-Pro gives no activity. rXpmA also shows no activity toward three peptides with proline at the N-terminus (Pro-Ala, Pro-Leu-Gly, and Pro-Leu-Ser-Arg-Tyr-Leu-Ser-Val-Ala-Ala-Lys-Lys) | Aspergillus oryzae ATCC 42149 | ? | - |
? | |
Phe-Pro + H2O | - |
Aspergillus oryzae | Phe + Pro | - |
? | |
Phe-Pro + H2O | - |
Aspergillus oryzae RIB 40 | Phe + Pro | - |
? | |
Phe-Pro + H2O | - |
Aspergillus oryzae ATCC 42149 | Phe + Pro | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 69000, recombinant His-tagged enzyme, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase P | - |
Aspergillus oryzae |
AMPP | - |
Aspergillus oryzae |
XpmA | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | purified recombinant enzyme, stable up to, inactivation at 60°C | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | - |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 12 | activity range, recombinant enzyme | Aspergillus oryzae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | purified recombinant enzyme, stable at. The enzyme maintains activity after incubation at pH 3.5 -12.0, pretreatment between pH 4.0 and pH 7.5 or between pH 8.5 and pH 11.0 results in enzyme activity above 100% of control, in particular at pH 4.0-5.5 about 4-5fold | Aspergillus oryzae |