Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Bos taurus | |
NaCl | above 0.25 M | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
Bos taurus | |
0.15 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe | - |
Bos taurus | |
0.15 | - |
Arg-Pro-Pro-Gly-Phe-Ser | - |
Bos taurus | |
0.25 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro | - |
Bos taurus | |
0.34 | - |
Arg-Pro-Pro-Gly-Phe | - |
Bos taurus | |
0.76 | - |
Arg-Pro-Pro | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | attached to the membranes via a glycosylphosphatidylinositol anchor | Bos taurus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
x * 95000, reducing conditions, SDS-PAGE | Bos taurus |
360000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | the enzyme is involved in the pulmonary inactivation of circulating bradykinin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | the enzyme is attached to the membranes via a glycosylphosphatidylinositol anchor | Bos taurus |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, pH 6.8, stable for at least 1 month | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Pro-Pro + H2O | - |
Bos taurus | Arg + Pro-Pro | - |
? | |
Arg-Pro-Pro-Gly-Phe + H2O | - |
Bos taurus | ? | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser + H2O | - |
Bos taurus | ? | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O | - |
Bos taurus | ? | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe + H2O | - |
Bos taurus | ? | - |
? | |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | i.e. bradykinin | Bos taurus | Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
? | |
additional information | the enzyme favors peptides with 2 proline residues or proline analogs in position 2 and 3 of the substrate | Bos taurus | ? | - |
? | |
additional information | the enzyme is involved in the pulmonary inactivation of circulating bradykinin | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | x * 95000, reducing conditions, SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 6.8, 30 min, stable up to | Bos taurus |
75 | - |
pH 6.8, 30 min, complete loss of activity | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
26 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
Bos taurus | |
30 | - |
Arg-Pro-Pro-Gly-Phe-Ser | - |
Bos taurus | |
39 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe | - |
Bos taurus | |
41 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro | - |
Bos taurus | |
48 | - |
Arg-Pro-Pro-Gly-Phe | - |
Bos taurus | |
120 | - |
Arg-Pro-Pro | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
hydrolysis of Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | Bos taurus |
7.5 | - |
- |
Bos taurus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 11 | 37°C, 2 h, maximal stability | Bos taurus |