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Literature summary for 3.4.11.9 extracted from

  • Orawski, A.T.; Simmons, W.H.
    Purification and properties of membrane-bound aminopeptidase P from rat lung (1995), Biochemistry, 34, 11227-11236.
    View publication on PubMed
Search for more literature for 3.4.11.9

General Stability

General Stability Organism
polyethylene glycol 20000 improves stability in the pH-range 4.5-7.0 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Rattus norvegicus
2-mercaptoethanol
-
 Rattus norvegicus
2-mercaptomethyl-3-guanidinoethylthiopropanoic acid
-
 Rattus norvegicus
4-chloromercuriphenyl sulfonic acid
-
 Rattus norvegicus
apstatin
-
 Rattus norvegicus
captopril
-
 Rattus norvegicus
Co2+ 1.5 mM CoCl2, complete inhibition Rattus norvegicus
dithiothreitol
-
 Rattus norvegicus
EDTA
-
 Rattus norvegicus
enalaprilat inhibition only in presence of Mn2+ Rattus norvegicus
N-[1-(R,S)-carboxy-(2-phenylethyl)]-thiopropanoic acid
-
 Rattus norvegicus
NaCl 2 M, complete inhibition Rattus norvegicus
ramiprilat inhibition only in presence of Mn2+ Rattus norvegicus
Zn2+ 10 mM, complete inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 bradykinin
-
 Rattus norvegicus
0.03
-
 L-Arg-L-Pro-L-Pro-Gly
-
 Rattus norvegicus
0.032
-
 Arg-Pro-Pro-Gly-Phe-Ser
-
 Rattus norvegicus
0.039
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
-
 Rattus norvegicus
0.048
-
 Arg-Pro-Pro-Gly-Phe
-
 Rattus norvegicus
0.051
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro
-
 Rattus norvegicus
0.15
-
 Arg-homoPro-Pro-Ala-NH2
-
 Rattus norvegicus
0.32
-
 Gly-Pro-Hyp without Mn2+ Rattus norvegicus
0.35
-
 Arg-Pro-Pro without Mn2+ Rattus norvegicus
0.36
-
 Arg-Pro-Pro in presence of 4 mM Mn2+ Rattus norvegicus
1
-
 Arg-Pro-Pro
-
 Rattus norvegicus
2
-
 Gly-Pro-Hyp in presence of 4 mM Mn2+ Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome membrane-bound Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.3-0.4 mM, 4fold stimulation with Gly-Pro-Hyp as substrate Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 x * 90000, SDS-PAGE Rattus norvegicus
220000
-
 value depends on assay conditions, gel filtration Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification the enzyme contains at least 17% N-linked carbohydrate Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allostatin 1 + H2O
-
 Rattus norvegicus Ala + ? cleavage of the Ala1-Pro2 bond ?
Arg-homoPro-Pro-Ala-NH2 + H2O
-
 Rattus norvegicus ?
-
 ?
Arg-Pro-Pro + H2O
-
 Rattus norvegicus Arg + Pro-Pro
-
 ?
Arg-Pro-Pro-Gly-Phe + H2O
-
 Rattus norvegicus ?
-
 ?
Arg-Pro-Pro-Gly-Phe-Ser + H2O
-
 Rattus norvegicus ?
-
 ?
Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O
-
 Rattus norvegicus ?
-
 ?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
-
 Rattus norvegicus Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 ?
Gly-Pro-Hyp + H2O
-
 Rattus norvegicus Gly + Pro-Hyp
-
 ?
L-Arg-L-Pro-L-Pro-Gly + H2O
-
 Rattus norvegicus L-Arg + L-Pro-L-Pro-Gly
-
 ?
additional information no cleavage of human erythropoietin Rattus norvegicus ?
-
 ?
neuropeptide Y + H2O
-
 Rattus norvegicus Tyr + ? cleavage of the Try1-Pro2 bond ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 pH 6.8, 30 min, 0.1 M potassium phosphate buffer, stable up to Rattus norvegicus
70 75 pH 6.8, 30 min, complete loss of activity Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 Arg-Pro-Pro-Gly-Phe-Ser
-
 Rattus norvegicus
12
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
 Rattus norvegicus
13
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe
-
 Rattus norvegicus
15
-
 Arg-Pro-Pro-Gly-Phe
-
 Rattus norvegicus
16
-
 Arg-Pro-Pro-Gly-Phe-Ser-Pro
-
 Rattus norvegicus
17
-
 L-Arg-L-Pro-L-Pro-Gly
-
 Rattus norvegicus
48
-
 Gly-Pro-Hyp
-
 Rattus norvegicus
69
-
 Gly-Pro-Hyp in presence of 4 mM Mn2+ Rattus norvegicus
150
-
 Arg-Pro-Pro
-
 Rattus norvegicus
180
-
 Arg-Pro-Pro in presence of 4 mM Mn2+ Rattus norvegicus
230
-
 Arg-Pro-Pro without 4 mM Mn2+ Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8 7.5 hydrolysis of Arg-Pro-Pro Rattus norvegicus
7
-
 hydrolysis of bradykinin(1-8), 0.5 mM, in both 0.1 M Hepes buffer or 0.1 M potassium phosphate buffer Rattus norvegicus
8
-
 6 mM imidazole buffer, Arg-Pro-Pro as substrate Rattus norvegicus
9
-
 11 mM imidazole buffer, hydrolysis of Arg-Pro-Pro Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 polyethylene glycol 20000 improves stability in the pH-range 4.5-7.0 Rattus norvegicus
7 10.5 optimal stability Rattus norvegicus
8.5 10 at 37°C, 2 h, most stable at Rattus norvegicus