Literature summary for 3.4.11.7 extracted from

Stressler, T.; Tanzer, C.; Ewert, J.; Claassen, W.; Fischer, L.
Simple purification method for a recombinantly expressed native His-tag-free aminopeptidase A from Lactobacillus delbrueckii (2017), Protein Expr. Purif., 131, 7-15 .

Search for more literature for 3.4.11.7

Cloned(Commentary)

Cloned (Comment)	Organism
gene pepA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)	Lactobacillus delbrueckii subsp. lactis

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	enzyme Lb-PepA is not product inhibited by glutamic acid	Lactobacillus delbrueckii subsp. lactis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	Michaelis-Menten kinetics	Lactobacillus delbrueckii subsp. lactis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Co2+	best activating metal ion	Lactobacillus delbrueckii subsp. lactis
Cu2+	activates, but less than Co2+	Lactobacillus delbrueckii subsp. lactis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
460000	480000	recombinant untagged enzyme, gel filtration	Lactobacillus delbrueckii subsp. lactis

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus delbrueckii subsp. lactis	F0HXE4	-	-
Lactobacillus delbrueckii subsp. lactis DSM 20072	F0HXE4	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant untagged enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, dialysis, and gel filtration to about 95% purity, method optimization	Lactobacillus delbrueckii subsp. lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.42	-	purified recombinant untagged enzyme, pH 6.0, 60°C, substrate L-Asp-4-nitroanilide	Lactobacillus delbrueckii subsp. lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Asp-4-nitroanilide + H2O	-	Lactobacillus delbrueckii subsp. lactis	L-Asp + 4-nitroaniline	-	?
L-Asp-4-nitroanilide + H2O	-	Lactobacillus delbrueckii subsp. lactis DSM 20072	L-Asp + 4-nitroaniline	-	?
L-Glu-4-nitroanilide + H2O	-	Lactobacillus delbrueckii subsp. lactis	L-Glu + 4-nitroaniline	-	?
L-Glu-4-nitroanilide + H2O	-	Lactobacillus delbrueckii subsp. lactis DSM 20072	L-Glu + 4-nitroaniline	-	?

Subunits

Subunits	Comment	Organism
homododecamer	12 * 40000, recombinant untagged enzyme, SDS-PAGE	Lactobacillus delbrueckii subsp. lactis

Synonyms

Synonyms	Comment	Organism
aminopeptidase A	-	Lactobacillus delbrueckii subsp. lactis
Lb-PepA	-	Lactobacillus delbrueckii subsp. lactis
PepA	-	Lactobacillus delbrueckii subsp. lactis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Lactobacillus delbrueckii subsp. lactis

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	80	4.7% of maximal activity at 30°C, 88% at 60°C, and 91% at 70°C, inactivation at 80°C	Lactobacillus delbrueckii subsp. lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
-	-	purified recombinant untagged enzyme, pH 7.5, stable for 7 days	Lactobacillus delbrueckii subsp. lactis
50	-	purified recombinant untagged enzyme, pH 7.5, 76% activity remaining after 1 h, inactivated after 24 h	Lactobacillus delbrueckii subsp. lactis
60	-	purified recombinant untagged enzyme, pH 7.5, inactivation within 1 h	Lactobacillus delbrueckii subsp. lactis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Lactobacillus delbrueckii subsp. lactis

pI Value

Organism	Comment	pI Value Maximum	pI Value
Lactobacillus delbrueckii subsp. lactis	sequence calculation	-	4.8
Lactobacillus delbrueckii subsp. lactis	recombinant untagged enzyme, isoelectric focusing	-	5.7

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Release 2023.1 (January 2023)