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Literature summary for 3.4.11.7 extracted from

  • Rozenfeld, R.; Iturrioz, X.; Maigret, B.; Llorens-Cortes, C.
    Contribution of molecular modeling and site-directed mutagenesis to the identification of two structural residues, Arg-220 and Asp-227, in aminopeptidase A (2002), J. Biol. Chem., 277, 29242-29252.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO cells Mus musculus

Protein Variants

Protein Variants Comment Organism
D227A mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking. Mutant enzyme shows 0.18% of the wild-type activity Mus musculus
D227R mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking Mus musculus
R220A mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking. Mutant enzyme shows 0.3% of the wild-type activity Mus musculus
R220D mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking Mus musculus
R220D/D227R mutant enzyme produces only the 140000 Da enzyme form by SDS-PAGE and autoradiography compared to a 168000 Da enzyme form and a 140000 Da enzyme form of the wild-type enzyme. Mutant enzyme displays incorrect maturation and trafficking Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
glutamate phosphonate 0.001 mM, activity of wild-type enzyme is completely abolished Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus P16406
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus