Cloned (Comment) | Organism |
---|---|
- |
Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
H450F | mutation markedly lowers the level of enzyme activity and changes the sensitivity to Ca2+, The EC50 for Ca2+ is 0.025 mM in the wild-type enzyme and 0.279 mM in the mutant enzyme. 15.5fold increase of KI-value for glutamate-thiol in presence of 0.025 mM Ca2+, 18.9fold increase of KI-value for glutamate-thiol in presence of 4 mM Ca2+, 91.8fold increase of Ki-value for glutamate phosphonic acid in presence of 0.025 mM Ca2+, 22.1fold increase of Ki-value for glutamate phosphonic acid in presence of 4 mM Ca2+, 2.5fold increase of Ki-value for lysine-thiol in presence of 0.025 mM Ca2+, 2.76fold increase of Ki-value for lysine-thiol in presence of 4 mM Ca2+. Mutant enzyme shows a 1150fold decrease in the ratio of turnover number to Km-value for alpha-L-Glu-beta-naphthylamide in presence of 0.025 mM Ca2+. Mutant enzyme shows a 571fold decrease in the ratio of turnover number to Km-value for alpha-L-Glu-beta-naphthylamide in presence of 5 mM Ca2+ | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glutamate phosphonic acid | - |
Mus musculus | |
glutamate-thiol | - |
Mus musculus | |
lysine-thiol | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.171 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, wild-type enzyme, in presence of 4 mM Ca2+ | Mus musculus | |
0.432 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, wild-type enzyme, in presence of 0.025 mM Ca2+ | Mus musculus | |
0.697 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, wild-type enzyme, without Ca2+ | Mus musculus | |
4.383 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, mutant enzyme H450F enzyme, in presence of 4 mM Ca2+ | Mus musculus | |
5.94 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, mutant enzyme H450F, in presence of 0.025 mM Ca2+ | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 7.3fold increase of ratio of turnover number to Km-value for the wild-type enzyme. Activity of mutant enzyme H450F is not measurable in absence of Ca2+. His450 together with Ca2+ may contribute to substrate specificity | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P16406 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-L-Glu-beta-naphthylamide + H2O | - |
Mus musculus | L-Glu + 2-naphthylamine | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, mutant enzyme H450F, in presence of 0.025 mM Ca2+ | Mus musculus | |
7.5 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, mutant enzyme H450F enzyme, in presence of 4 mM Ca2+ | Mus musculus | |
93 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, wild-type enzyme, without Ca2+ | Mus musculus | |
149 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, wild-type enzyme, in presence of 0.025 mM Ca2+ | Mus musculus | |
166 | - |
alpha-L-Glu-beta-naphthylamide | pH 7.4, 37°C, wild-type enzyme, in presence of 4 mM Ca2+ | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000038 | - |
glutamate phosphonic acid | pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+ | Mus musculus | |
0.00012 | - |
glutamate-thiol | pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+ | Mus musculus | |
0.00036 | - |
glutamate-thiol | pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+ | Mus musculus | |
0.00046 | - |
lysine-thiol | pH 7.4, 37°C, wild-type enzyme, Ca2+ | Mus musculus | |
0.00047 | - |
lysine-thiol | pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+ | Mus musculus | |
0.00061 | - |
glutamate phosphonic acid | pH 7.4, 37°C, wild-type enzyme, 0.025 mM Ca2+ | Mus musculus | |
0.00084 | - |
glutamate phosphonic acid | pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+ | Mus musculus | |
0.0012 | - |
lysine-thiol | pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+ | Mus musculus | |
0.00167 | - |
glutamate-thiol | pH 7.4, 37°C, wild-type enzyme, no Ca2+ | Mus musculus | |
0.00227 | - |
glutamate-thiol | pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+ | Mus musculus | |
0.00287 | - |
glutamate phosphonic acid | pH 7.4, 37°C, wild-type enzyme, no Ca2+ | Mus musculus | |
0.00524 | - |
glutamate-thiol | pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+ | Mus musculus | |
0.0072 | - |
lysine-thiol | pH 7.4, 37°C, wild-type enzyme, 4 mM Ca2+ | Mus musculus | |
0.0199 | - |
lysine-thiol | pH 7.4, 37°C, mutant enzyme H450F, 4 mM Ca2+ | Mus musculus | |
0.056 | - |
glutamate phosphonic acid | pH 7.4, 37°C, mutant enzyme H450F, 0.025 mM Ca2+ | Mus musculus |