Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.11.6 extracted from

  • Cadel, S.; Darmon, C.; Desert, A.; Mahbouli, M.; Piesse, C.; Ghelis, T.; Lafont, R.; Foulon, T.
    The effects of curcumin, mangiferin, resveratrol and other natural plant products on aminopeptidase B activity (2019), Biochem. Biophys. Res. Commun., 512, 832-837 .
    View publication on PubMed
Search for more literature for 3.4.11.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in baculovirus infected SF9 insect cells Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
curcumin non-competitive inhibitor Rattus norvegicus
mangiferin mixed non-competitive inhibitor Rattus norvegicus
additional information At 0.2 mM, no significant inhibitory effect is observed with caffeic, chlorogenic, ferulic, salicylic and sinapic acids as well as with resveratrol Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
 L-Arg-7-amido-4-methylcoumarin at pH 7.4 and 28°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography and Superdex S200 gel filtration Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Arg-7-amido-4-methylcoumarin + H2O
-
 Rattus norvegicus L-Arg + 7-amino-4-methylcoumarin
-
 ?
L-Arg-L-Tyr-Gly-Gly-L-Phe-L-Leu + H2O
-
 Rattus norvegicus L-Arg + L-Tyr-Gly-Gly-L-Phe-L-Leu
-
 ?

Synonyms

Synonyms Comment Organism
Ap-B
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
43
-
 L-Arg-7-amido-4-methylcoumarin at pH 7.4 and 28°C Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.046
-
 curcumin at pH 7.4 and 28°C Rattus norvegicus
0.105
-
 mangiferin at pH 7.4 and 28°C Rattus norvegicus