Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
Y229H | Km and kcat (L-Arg-2-naphthylamide) decreased compared to wild-type | Rattus norvegicus |
Y409F | Km and kcat (L-Arg-2-naphthylamide) decreased compared to wild-type | Rattus norvegicus |
Y441F | Km and kcat (L-Arg-2-naphthylamide) decreased compared to wild-type | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arphamenine A | - |
Rattus norvegicus | |
Arphamenine B | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0453 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y441F | Rattus norvegicus | |
0.06 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y229H | Rattus norvegicus | |
0.0987 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y409F | Rattus norvegicus | |
0.162 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, wild-type | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Leu-enkephalin + H2O | - |
Rattus norvegicus | Arg + Leu-enkephalin | - |
? | |
L-Arg-2-naphthylamide + H2O | - |
Rattus norvegicus | L-Arg + 2-naphthylamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aminopeptidase B | - |
Rattus norvegicus |
Ap-B | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y441F | Rattus norvegicus | |
4.8 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y409F | Rattus norvegicus | |
9.4 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y229H | Rattus norvegicus | |
25 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, wild-type | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000076 | - |
arphamenine A | pH 7.4, 37°C, mutant Y229H | Rattus norvegicus | |
0.0000085 | - |
arphamenine A | pH 7.4, 37°C, wild-type | Rattus norvegicus | |
0.0000085 | - |
Arphamenine B | pH 7.4, 37°C, mutant Y229H | Rattus norvegicus | |
0.0000103 | - |
Arphamenine B | pH 7.4, 37°C, wild-type | Rattus norvegicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y409F | Rattus norvegicus | |
51 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y441F | Rattus norvegicus | |
154 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, wild-type | Rattus norvegicus | |
157 | - |
L-Arg-2-naphthylamide | pH 7.4, 37°C, mutant Y229H | Rattus norvegicus |