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Literature summary for 3.4.11.6 extracted from
- Purification and characterization of aminopeptidase B from goat brain (2009), Process Biochem., 44, 776-780.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Capra aegagrus |  |
| Mn2+ | - |
Capra aegagrus | |
| Sn2+ | - |
Capra aegagrus | |
| Zn2+ | - |
Capra aegagrus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Capra aegagrus | |
| 5,5 dithiobis(2-nitrobenzoic acid) | 1 mM, 74.89% enzyme inhibition | Capra aegagrus | |
| Cd2+ | - |
Capra aegagrus | |
| DMSO | - |
Capra aegagrus | |
| DTT | 1 mM, complete inhibition of enzyme activity | Capra aegagrus | |
| EDTA | 1 mM, 42.33% enzyme inhibition | Capra aegagrus | |
| ethanol | - |
Capra aegagrus | |
| Fe2+ | - |
Capra aegagrus | |
| Hg2+ | - |
Capra aegagrus | |
| Ni2+ | - |
Capra aegagrus | |
| p-aminophenylmercuric acetate | 1 mM, 41.27% enzyme inhibition | Capra aegagrus | |
| p-hydroxymercuriphenyl sulfonic acid | 1 mM, 72.22% enzyme inhibition | Capra aegagrus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
N-naphthalen-2-yl-L-argininamide | - |
Capra aegagrus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | inhibitor | Capra aegagrus | |
| Co2+ | slight enzyme activation | Capra aegagrus | |
| Fe2+ | inhibitor | Capra aegagrus | |
| Hg2+ | inhibitor | Capra aegagrus | |
| Mn2+ | slight enzyme activation | Capra aegagrus | |
| Ni2+ | inhibitor | Capra aegagrus | |
| Sn2+ | slight enzyme activation | Capra aegagrus | |
| Zn2+ | slight enzyme activation | Capra aegagrus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79700 | - |
SDS-PAGE | Capra aegagrus |
| 80200 | - |
Sephadex G-200 | Capra aegagrus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | at 1.5 M urea only 10% remaining enzyme acitvity, at 2.0 M urea complete loss of enzyme activity | Capra aegagrus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Capra aegagrus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 279.5fold after heat inactivation, ammonium sulfate fractionation and column chromatographies | Capra aegagrus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Capra aegagrus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arginine-2-naphthylamide + H2O | assay at 37°C | Capra aegagrus | 2-naphthylamine + L-Arg | - |
? | |
| L-lysine-2-naphthylamide + H2O | assay at 37°C | Capra aegagrus | 2-naphthylamine + L-Lys | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Capra aegagrus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aminopeptidase B | - |
Capra aegagrus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Capra aegagrus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Capra aegagrus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
1,10-phenanthroline | - |
Capra aegagrus | |
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