Literature summary for 3.4.11.6 extracted from

Hui, M.; Hui, K.S.
A novel aminopeptidase with highest preference for lysine (2006), Neurochem. Res., 31, 95-102.

Activating Compound

Activating Compound	Comment	Organism	Structure
NaF	0.4 M activates the activity by 20%	Rattus norvegicus

Inhibitors

Inhibitors	Comment	Organism
1,10-phenanthroline	34% residual activity at 1 mM	Rattus norvegicus
actinonin	-	Rattus norvegicus
Aprotinin	34% residual activity at 0.25 mg/ml	Rattus norvegicus
arphamemine B	-	Rattus norvegicus
bestatin	highly sensitive to bestatin	Rattus norvegicus
Ca2+	19% inhibition at 1 mM	Rattus norvegicus
Cd2+	complete inhibition at 1 mM	Rattus norvegicus
Co2+	76% inhibition at 1 mM	Rattus norvegicus
Cu2+	complete inhibition at 1 mM	Rattus norvegicus
E-64	99% residual activity at 0.1 mM	Rattus norvegicus
EDTA	61% residual activity at 1 mM	Rattus norvegicus
EGTA	complete inhibition at 1 mM	Rattus norvegicus
Hg2+	complete inhibition at 1 mM	Rattus norvegicus
Leucine hydroxamate	-	Rattus norvegicus
leucinthiol	-	Rattus norvegicus
Mn2+	82% inhibition at 1 mM	Rattus norvegicus
additional information	insensitive to L-Arg-hydroxamate, L-Lys-hydroxamate, puromycin, and amastatin	Rattus norvegicus
Na+	NaCl (0.1-100 mM) has an opposite effect on the EGTA-treated KAP apo-enzyme, it inhibits 13% at 0.1 mM and 100% at 100 mM	Rattus norvegicus
Ni2+	complete inhibition at 1 mM	Rattus norvegicus
Pb2+	84% inhibition at 1 mM	Rattus norvegicus
pepstatin	93% residual activity at 0.1 mM	Rattus norvegicus
Phenylmethanesulfonylfluoride	45% residual activity at 0.5 mM	Rattus norvegicus
Zn2+	80% inhibition at 1 mM	Rattus norvegicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.333	-	L-Lys-2-naphthylamide	-	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-
membrane	synaptic membrane	Rattus norvegicus	16020	-

Metals/Ions

Metals/Ions	Comment	Organism
K+	0.4 M activates the activity by 55%	Rattus norvegicus
Li+	0.4 M activates the activity by 45%	Rattus norvegicus
Na+	0.4 M activates the activity by 190%	Rattus norvegicus
Zn2+	Zn2+-exopeptidase	Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62000	-	gel filtration	Rattus norvegicus

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Toyopearl DEAE-650 column chromatography, Mono Q column chromatography, and DEAE-650 column chromatography	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
adrenal gland	-	Rattus norvegicus	-
brain	-	Rattus norvegicus	-
cerebellum	-	Rattus norvegicus	-
cerebral cortex	-	Rattus norvegicus	-
hippocampus	-	Rattus norvegicus	-
hypothalamus	-	Rattus norvegicus	-
intestine	-	Rattus norvegicus	-
kidney	-	Rattus norvegicus	-
liver	-	Rattus norvegicus	-
medulla oblongata	-	Rattus norvegicus	-
midbrain	-	Rattus norvegicus	-
muscle	-	Rattus norvegicus	-
pancreas	-	Rattus norvegicus	-
pituitary gland	-	Rattus norvegicus	-
serum	-	Rattus norvegicus	-
spleen	-	Rattus norvegicus	-
testis	-	Rattus norvegicus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
Gly-2-naphthylamide + H2O	1% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	Gly + 2-naphthylamine	-	?
Gly-L-Pro-2-naphthylamide + H2O	1% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	Gly-L-Pro + 2-naphthylamine	-	?
L-Ala-2-naphthylamide + H2O	83% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-Ala + 2-naphthylamine	-	?
L-Arg-2-naphthylamide + H2O	17% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-Arg + 2-naphthylamine	-	?
L-beta-Asn-2-naphthylamide + H2O	9% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-beta-Asn + 2-naphthylamine	-	?
L-Cys-2-naphthylamide + H2O	1% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-Cys + 2-naphthylamine	-	?
L-Gly-L-Arg-2-naphthylamide + H2O	250% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-Gly-L-Arg + 2-naphthylamine	-	?
L-His-2-naphthylamide + H2O	15% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-His + 2-naphthylamine	-	?
L-Leu-2-naphthylamide + H2O	31% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-Leu + 2-naphthylamine	-	?
L-Lys-2-naphthylamide + H2O	100% activity	Rattus norvegicus	L-Lys + 2-naphthylamine	-	?
L-Lys-L-Ala-2-naphthylamide + H2O	7% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-Lys-L-Ala + 2-naphthylamine	-	?
L-Met-2-naphthylamide + H2O	2% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-Met + 2-naphthylamine	-	?
L-Tyr-2-naphthylamide + H2O	62% activity compared to L-Lys-2-naphthylamide	Rattus norvegicus	L-Tyr + 2-naphthylamine	-	?
additional information	no activity with L-alpha-Asn-2-naphthylamide, L-Glu-2-naphthylamide, L-Ile-2-naphthylamide, L-Phe-2-naphthylamide, L-Pro-2-naphthylamide, L-Ser-2-naphthylamide, L-Thr-2-naphthylamide, L-Trp-2-naphthylamide, L-Val-2-naphthylamide, and L-Arg-L-Arg-2-naphthylamide	Rattus norvegicus	?	-	?

Synonyms

Synonyms	Comment	Organism
aminopeptidase B	-	Rattus norvegicus
APB	-	Rattus norvegicus
KAP	-	Rattus norvegicus
lysine-specific aminopeptidase	-	Rattus norvegicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Rattus norvegicus

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	9	-	Rattus norvegicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism
0.00021	-	bestatin	at 37°C in Bicine buffer complemented with 0.2 M NaCl	Rattus norvegicus
0.00054	-	leucinthiol	at 37°C in Bicine buffer complemented with 0.2 M NaCl	Rattus norvegicus
0.0125	-	arphamemine B	at 37°C in Bicine buffer complemented with 0.2 M NaCl	Rattus norvegicus
0.02	-	actinonin	at 37°C in Bicine buffer complemented with 0.2 M NaCl	Rattus norvegicus
0.024	-	Leucine hydroxamate	at 37°C in Bicine buffer complemented with 0.2 M NaCl	Rattus norvegicus