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Literature summary for 3.4.11.6 extracted from

  • Hiraoka, B.Y.; Fukasawa, K.; Harada, M.
    Purification and characterization of two novel arginine aminopeptidases from Streptococcus mitis ATCC 9811 (1983), J. Biochem., 94, 1201-1208.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol activation, enzyme II Streptococcus mitis
EDTA activation Streptococcus mitis
sulfhydryl compounds activation, enzyme I Streptococcus mitis

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol enzyme II Streptococcus mitis
Cd2+
-
Streptococcus mitis
Co2+
-
Streptococcus mitis
Cu2+
-
Streptococcus mitis
cysteine enzyme II Streptococcus mitis
Hg2+
-
Streptococcus mitis
p-chloromercuribenzoate
-
Streptococcus mitis
Pb2+
-
Streptococcus mitis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
L-Arg-beta-naphthylamide enzyme I Streptococcus mitis
0.038
-
L-Arg-beta-naphthylamide enzyme II Streptococcus mitis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000 58000 gel filtration, enzyme I Streptococcus mitis
360000
-
gel filtration, enzyme II Streptococcus mitis

Organism

Organism UniProt Comment Textmining
Streptococcus mitis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 forms Streptococcus mitis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Streptococcus mitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Arg-Gly-Tyr-Ala-Leu-Gly + H2O
-
Streptococcus mitis Arg + Gly-Tyr-Ala-Leu-Gly
-
?
Arg-Tyr-Leu-Pro-Thr + H2O proctolin Streptococcus mitis Arg + Tyr-Leu-Pro-Thr
-
?
Arg-Val-Tyr-Ile-His-Pro-Phe + H2O angiotensin III Streptococcus mitis Arg + Val-Tyr-Ile-His-Pro-Phe
-
?
L-Arg-2-naphthylamide + H2O
-
Streptococcus mitis L-Arg + 2-naphthylamine
-
?

Subunits

Subunits Comment Organism
monomer 1 * ?, enzyme I, SDS-PAGE Streptococcus mitis