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Literature summary for 3.4.11.5 extracted from

  • Li, N.; Wu, J.M.; Zhang, L.F.; Zhang, Y.Z.; Feng, H.
    Characterization of a unique proline iminopeptidase from white-rot basidiomycetes Phanerochaete chrysosporium (2010), Biochimie, 92, 779-788 .
    View publication on PubMed
Search for more literature for 3.4.11.5

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Phanerodontia chrysosporium

Protein Variants

Protein Variants Comment Organism
D264A mutation in educed catalytic residue, complete inactivation Phanerodontia chrysosporium
D264H mutation in educed catalytic residue, complete inactivation Phanerodontia chrysosporium
S107D mutation in educed catalytic residue, complete inactivation Phanerodontia chrysosporium

Inhibitors

Inhibitors Comment Organism Structure
Benzamidine hydrochloride 5 mM, 47.6% residual activity Phanerodontia chrysosporium
Cu2+ 1 mM, 3.1% residual activity Phanerodontia chrysosporium
EDTA 5 mM, 85.7% residual activity Phanerodontia chrysosporium
Hg2+ 1 mM, 0.8% residual activity Phanerodontia chrysosporium
additional information not inhibitory: dithiothreitol, 2-mercaptoethanol, H2O2 Phanerodontia chrysosporium
phenylmethylsulfonyl fluoride 5 mM, 43.4% residual activity Phanerodontia chrysosporium
Zn2+ 1 mM, 1.2% residual activity Phanerodontia chrysosporium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.15
-
 L-Pro-4-nitroanilide pH 7.5, 45°C Phanerodontia chrysosporium

Organism

Organism UniProt Comment Textmining
Phanerodontia chrysosporium C6KI04
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Pro-4-nitroanilide + H2O
-
 Phanerodontia chrysosporium L-Pro + 4-nitroaniline
-
 ?
additional information enzyme releases L-lysine from various hydrolytic products of milk, BSA and collagen treated with the alkaline protease DHAP. No substrates: L-Leu-4-nitroanilide, L-Phe-4-nitroanilide, L-Tyr-4-nitroanilide Gly-Gly-Gly-4-nitroanilide, L-Ser-Gly-L-Arg-4-nitroanilide, L-Ala-L-Ala-L-Val4-nitroanilide, L-Ala-L-Ala-L-Pro-L-Leu-4-nitroanilide, L-Ala-L-Ala-L-Val-L-Ala-4-nitroanilide Phanerodontia chrysosporium ?
-
 ?

Subunits

Subunits Comment Organism
? c * 38000, SDS-PAGE, x * 37619, calculated Phanerodontia chrysosporium

Synonyms

Synonyms Comment Organism
PiPA
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Phanerodontia chrysosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 stable for 30 min Phanerodontia chrysosporium
55
-
 10 min, complete loss of activity Phanerodontia chrysosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
218
-
 L-Pro-4-nitroanilide pH 7.5, 45°C Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Phanerodontia chrysosporium

pH Range

pH Minimum pH Maximum Comment Organism
5 11
-
 Phanerodontia chrysosporium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
104
-
 L-Pro-4-nitroanilide pH 7.5, 45°C Phanerodontia chrysosporium