Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 as a His-tagged fusion protein | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PMSF | - |
Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
L-Pro-4-nitroanilide | pH 7.5, 60°C | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits activity | Aspergillus oryzae | |
Ni2+ | enhances activity | Aspergillus oryzae | |
Zn2+ | inhibits activity | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Collagen + H2O | - |
Aspergillus oryzae | ? | - |
? | |
L-Pro-4-nitroanilide + H2O | using ten different aminoacyl-pNA substrates. PAP only shows catalytic activity toward L-Pro-4-nitroanilide | Aspergillus oryzae | L-Pro + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Aspergillus oryzae |
proline-specific aminopeptidase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus oryzae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
after 1 h enzyme still maintains an activity of 80% | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Aspergillus oryzae |