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Literature summary for 3.4.11.5 extracted from
- Over-expression of a proline specific aminopeptidase from Aspergillus oryzae JN-412 and its application in collagen degradation (2014), Appl. Biochem. Biotechnol., 173, 1765-1777.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 as a His-tagged fusion protein | Aspergillus oryzae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PMSF | - |
Aspergillus oryzae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
L-Pro-4-nitroanilide | pH 7.5, 60°C | Aspergillus oryzae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibits activity | Aspergillus oryzae | |
| Ni2+ | enhances activity | Aspergillus oryzae | |
| Zn2+ | inhibits activity | Aspergillus oryzae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA chromatography | Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Collagen + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| L-Pro-4-nitroanilide + H2O | using ten different aminoacyl-pNA substrates. PAP only shows catalytic activity toward L-Pro-4-nitroanilide | Aspergillus oryzae | L-Pro + 4-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PAP | - |
Aspergillus oryzae |
| proline-specific aminopeptidase | - |
Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
after 1 h enzyme still maintains an activity of 80% | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Aspergillus oryzae |
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Release 2023.1 (January 2023)
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