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Literature summary for 3.4.11.5 extracted from

  • Marinova, M.; Dolashki, A.; Altenberend, F.; Stevanovic, S.; Voelter, W.; Tchorbanov, B.
    Characterization of an aminopeptidase and a proline iminopeptidase from cabbage leaves (2008), Z. Naturforsch. C, 63, 105-112.
    View publication on PubMed
Search for more literature for 3.4.11.5

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Brassica oleracea
ethanol strong inhibitor Brassica oleracea
additional information EDTA, Mg2+, or Ca2+ show no or little inhibitory effect Brassica oleracea
Na+ slight inhibition at 2 mM NaCl Brassica oleracea
p-chloromercuribenzoic acid
-
 Brassica oleracea
phenylmethylsulfonyl fluoride
-
 Brassica oleracea
Urea
-
 Brassica oleracea
Zn2+
-
 Brassica oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, SDS-PAGE Brassica oleracea
56000
-
 4 * 56000, gel filtration Brassica oleracea
204000
-
 gel filtration Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
 Brassica oleracea var. capitata
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, Toyopearl QAE-550C column chromatography, Sephadex G-200 gel filtration, and Mono Q column chromatography Brassica oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
 crude extract, in 50 mM Tris-HCl, pH 7.5, at 37°C Brassica oleracea
10
-
 after 700fold purification, in 50 mM Tris-HCl, pH 7.5, at 37°C Brassica oleracea

Storage Stability

Storage Stability Organism
4°C, in 50 mM Tris-HCl buffer at pH 7.5, several months, no loss of activity Brassica oleracea
4°C, in phosphate buffer between pH 7.5 and 8.5, overnight, remains stable Brassica oleracea
4°C, in the absence of protective reagents, 3 days, little or no activity remains Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-prolyl-p-nitroanilide + H2O
-
 Brassica oleracea L-proline + p-nitroaniline
-
 ?
additional information not active with L-Phe-p-nitroanilide, L-Ala-p-nitroanilide, L-Leu-p-nitroanilide, casein, and benzyl-Arg-p-nitroanilide Brassica oleracea ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 50000, SDS-PAGE Brassica oleracea
homotetramer 4 * 56000, gel filtration Brassica oleracea

Synonyms

Synonyms Comment Organism
proline iminopeptidase
-
 Brassica oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Brassica oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme starts to become labile at 50°C, after 120 min at 50°C 50% of its initial activity is lost Brassica oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Brassica oleracea