Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | restores activity after deactivation with p-chloromercuribenzoate | Priestia megaterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Priestia megaterium | |
Hg2+ | - |
Priestia megaterium | |
p-chloromercuribenzoate | - |
Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.152 | - |
L-Pro-2-naphthylamide | - |
Priestia megaterium | |
0.32 | - |
L-Pro-4-nitroanilide | - |
Priestia megaterium | |
0.78 | - |
Pro-D-Ala | - |
Priestia megaterium | |
0.83 | - |
Pro-Trp | - |
Priestia megaterium | |
0.9 | - |
Pro-Asp | - |
Priestia megaterium | |
1.08 | - |
Pro-Phe | - |
Priestia megaterium | |
1.15 | - |
Pro-Gly | - |
Priestia megaterium | |
1.55 | - |
Pro-Lys | - |
Priestia megaterium | |
1.86 | - |
Pro-D-Phe | - |
Priestia megaterium | |
2.69 | - |
Pro-Ala | - |
Priestia megaterium | |
3.6 | - |
L-Pro-L-Leu-Gly-NH2 | - |
Priestia megaterium | |
5.3 | - |
Pro-Leu | - |
Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration, SDS-PAGE | Priestia megaterium |
60000 | - |
1 * 60000, SDS-PAGE | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
var. polyliminolyticus | - |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
5°C | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Pro-2-naphthylamide + H2O | - |
Priestia megaterium | L-Pro + 2-naphthylamine | - |
? | |
L-Pro-4-nitroanilide + H2O | - |
Priestia megaterium | L-Pro + 4-nitroaniline | - |
? | |
L-Pro-L-Leu-Gly-NH2 + H2O | - |
Priestia megaterium | L-Pro + L-Leu-Gly-NH2 | - |
? | |
L-prolyl-peptide + H2O | cleavage of D-Pro residues | Priestia megaterium | L-proline + peptide | - |
? | |
L-prolyl-peptide + H2O | influence of amino acid adjacent to N-terminal L-Pro on rate of hydrolysis | Priestia megaterium | L-proline + peptide | - |
? | |
N-L-Pro-2-naphthylamide + H2O | - |
Priestia megaterium | L-proline + naphthylamine | - |
? | |
Pro-Ala + H2O | - |
Priestia megaterium | Pro + Ala | - |
? | |
Pro-Asp + H2O | - |
Priestia megaterium | Pro + Asp | - |
? | |
Pro-D-Ala + H2O | - |
Priestia megaterium | Pro + D-Ala | - |
? | |
Pro-D-Phe + H2O | - |
Priestia megaterium | Pro + D-Phe | - |
? | |
Pro-Gly + H2O | - |
Priestia megaterium | Pro + Gly | - |
? | |
Pro-Leu + H2O | - |
Priestia megaterium | Pro + Leu | - |
? | |
Pro-Lys + H2O | - |
Priestia megaterium | Pro + Lys | - |
? | |
Pro-Phe + H2O | - |
Priestia megaterium | Pro + Phe | - |
? | |
Pro-Trp + H2O | - |
Priestia megaterium | Pro + Trp | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7, 30 min, 50% activity | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Priestia megaterium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Priestia megaterium |