Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain M15 | Porphyromonas gingivalis |
Protein Variants | Comment | Organism |
---|---|---|
S603A | inactive | Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
Gly-Ala-Pro-beta-naphthylamide | in 20 mM Tris-HCl buffer (pH 7.0), at 37°C | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
2 * 77000, SDS-PAGE | Porphyromonas gingivalis |
145000 | - |
gel filtration | Porphyromonas gingivalis |
154000 | - |
SDS-PAGE | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Serratia marcescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Toyopearl HW65C column chromatography and DEAE-Toyopearl column chromatography | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Phe-Pro-beta-naphthylamide + H2O | - |
Serratia marcescens | Ala-Phe-Pro + beta-naphthylamine | - |
? | |
Ala-Phe-Pro-beta-naphthylamide + H2O | - |
Porphyromonas gingivalis | Ala-Phe-Pro + beta-naphthylamine | - |
? | |
Gly-Ala-Pro-beta-naphthylamide + H2O | - |
Serratia marcescens | Gly-Ala-Pro + beta-naphthylamine | - |
? | |
additional information | no activity with Gly-Pro-beta-naphthylamide, Z-Gly-Pro-beta-naphthylamide, Z-Gly-Ala-Pro-beta-naphthylamide, Z-Ala-Gly-Pro-beta-naphthylamide, Gly-Ala-Gly-Pro-beta-naphthylamide, Z-Gly-Ala-Gly-Pro-beta-naphthylamide, and Ala-Gly-Ala-Gly-Pro-beta-naphthylamide | Serratia marcescens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 77000, SDS-PAGE | Porphyromonas gingivalis |
Synonyms | Comment | Organism |
---|---|---|
prolyl tripeptidyl aminopeptidase | - |
Serratia marcescens |
prolyl tripeptidyl aminopeptidase | - |
Porphyromonas gingivalis |
PTP | - |
Serratia marcescens |
PTP | - |
Porphyromonas gingivalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Serratia marcescens |
42 | - |
- |
Porphyromonas gingivalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
354 | - |
Gly-Ala-Pro-beta-naphthylamide | in 20 mM Tris-HCl buffer (pH 7.0), at 37°C | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Serratia marcescens |
7.2 | - |
- |
Porphyromonas gingivalis |