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Literature summary for 3.4.11.4 extracted from

  • Walus, M.; Kida, E.; Wisniewski, K.E.; Golabek, A.A.
    Ser475, Glu272, Asp276, Asp327, and Asp360 are involved in catalytic activity of human tripeptidyl-peptidase I (2005), FEBS Lett., 579, 1383-1388.
    View publication on PubMed
Search for more literature for 3.4.11.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in CHO cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D276A kcat/KM is 21% of wild-type value Homo sapiens
D327A kcat/KM is 6% of wild-type value Homo sapiens
D360A kcat/KM is 3% of wild-type value Homo sapiens
E272A kcat/KM is 3% of wild-type value Homo sapiens
S475L kcat/KM is 0.4% of wild-type value Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.076
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme S475A Homo sapiens
0.098
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, wild-type enzyme Homo sapiens
0.105
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme D276A Homo sapiens
0.111
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme D360A Homo sapiens
0.118
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme E272A Homo sapiens
0.164
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme D327A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
TPP I
-
 Homo sapiens
tripeptidyl-peptidase I
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04 1.97 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme D276A Homo sapiens
0.12
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme S475A Homo sapiens
1.52
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme D360A Homo sapiens
1.54
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme E272A Homo sapiens
3.22
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, wild-type enzyme Homo sapiens
4.22
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme D327A Homo sapiens
9.36
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, mutant enzyme D276A Homo sapiens
41.66
-
 Ala-Ala-Phe-7-amido-4-methylcoumarin pH 5.0, 37°C, wild-type enzyme Homo sapiens