Any feedback?
Literature summary for 3.4.11.3 extracted from
- Mutational analysis of the active site of human insulin-regulated aminopeptidase (2001), Eur. J. Biochem., 268, 98-104.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in HEK293 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A429G | the ratio of maximal velocity to Km-value is 75% of the value for the wild-type enzyme | Homo sapiens |
| E431A | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
| E431D | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
| E465D | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
| E465Q | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
| E487D | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
| E487Q | inactive mutant enzyme | Homo sapiens |
| G428A | the ratio of maximal velocity to Km-value is 90% of the value for the wild-type enzyme | Homo sapiens |
| H464Y | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
| H468Y | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
| M430E | strong decrease in aminopeptidase activity towards Leu-4-nitroanilide | Homo sapiens |
| M430I | the ratio of maximal velocity to Km-value is 6.2% of the value for the wild-type enzyme | Homo sapiens |
| M430K | the ratio of maximal velocity to Km-value is 3.3% of the value for the wild-type enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.243 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, mutant enzyme A429G | Homo sapiens |  |
| 0.331 | - |
Cys-4-nitroanilide | pH 7.4, 37°C, wild-type enzyme | Homo sapiens | |
| 0.4567 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, mutant enzyme M430I | Homo sapiens | |
| 0.488 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, wild-type enzyme | Homo sapiens | |
| 0.529 | - |
Arg-4-nitroanilide | pH 7.4, 37°C, wild-type enzyme | Homo sapiens | |
| 0.593 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, mutant enzyme G428A | Homo sapiens | |
| 0.9148 | - |
Leu-4-nitroanilide | pH 7.4, 37°C, mutant enzyme M430K | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | contains the characteristic Zn2+-coordination sequence element, HEXXH-(18-64X)-E | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Arg-4-nitroanilide + H2O | - |
Homo sapiens | Arg + 4-nitroaniline | - |
? | |
| Cys-4-nitroanilide + H2O | - |
Homo sapiens | Cys + 4-nitroaniline | - |
? | |
| Leu-4-nitroanilide + H2O | - |
Homo sapiens | Leu + 4-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| insulin-regulated aminopeptidase | - |
Homo sapiens |
| IRAP | - |
Homo sapiens |
| oxytocinase | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
