Cloned (Comment) | Organism |
---|---|
leucine aminopeptidase/oxytocinase, expression in Chinese hamster ovary cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.1 mM, 83% inhibition. 0.01 mM, 10% inhibition | Homo sapiens | |
amastatin | 0.1 mM, 96% inhibition. 0.01 mM, 79% inhibition | Homo sapiens | |
bestatin | 0.1 mM, 43% inhibition. 0.01 mM, 12% inhibition | Homo sapiens | |
CuCl2 | 0.1 mM, 91% inhibition. 0.01 mM, 87% inhibition | Homo sapiens | |
leupeptin | 0.1 mM, 53% inhibition | Homo sapiens | |
additional information | EDTA has no effect | Homo sapiens | |
pepstatin A | 0.1 mM, 70% inhibition | Homo sapiens | |
puromycin | 0.1 mM, 36% inhibition. 0.01 mM, 5% inhibition | Homo sapiens | |
ZnCl2 | 0.1 mM, 98% inhibition. 0.01 mM, 77% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0225 | - |
Leu-4-methylcoumaryl-7-amide | pH 7.5, 37°C | Homo sapiens | |
0.0436 | - |
S-benzyl-Cys-4-methylcoumaryl-7-amide | pH 7.5, 37°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Homo sapiens | 16020 | - |
soluble | recombinant enzyme expressed in Chinese hamster ovary cells | Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | enzyme contains 1 mol of zinc per mol of enzyme, contains the consensus HEXXH(X)18E motif of zinc-metallopeptidase proteins | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
2 * 170000, SDS-PAGE | Homo sapiens |
280000 | - |
sedimentation velocity experiments | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
placenta | - |
Homo sapiens | - |
serum | serum level of the enzyme increases with gestation | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | Arg + 7-amino-4-methylcoumarin | - |
? | |
Leu-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | Leu + 7-amino-4-methylcoumarin | - |
? | |
Lys-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | Lys + 7-amino-4-methylcoumarin | - |
? | |
Met-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | Met + 7-amino-4-methylcoumarin | - |
? | |
S-benzyl-Cys-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | S-benzyl-Cys + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 170000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
leucine aminopeptidase/oxytocinase | - |
Homo sapiens |
P-LAP | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | 1.97 | Leu-4-methylcoumaryl-7-amide | pH 7.5, 37°C | Homo sapiens | |
9.18 | - |
Leu-4-methylcoumaryl-7-amide | pH 7.5, 37°C | Homo sapiens | |
12.4 | - |
S-benzyl-Cys-4-methylcoumaryl-7-amide | pH 7.5, 37°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
amastatin | pH 7.5, 37°C | Homo sapiens |