BRENDA - Enzyme Database show
show all sequences of 3.4.11.25

A novel beta-peptidyl aminopeptidase (BapA) from strain 3-2W4 cleaves peptide bonds of synthetic beta-tri- and beta-dipeptides

Geueke, B.; Namoto, K.; Seebach, D.; Kohler, H.P.; J. Bacteriol. 187, 5910-5917 (2005)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
Sphingosinicella xenopeptidilytica
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibitory effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2, CuCl2, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
Sphingosinicella xenopeptidilytica
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2 or CuCl2
Sphingosinicella xenopeptidilytica
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12000
-
x * 12000 + x * 27000, SDS-PAGE
Sphingosinicella xenopeptidilytica
27000
-
x * 12000 + x * 27000, SDS-PAGE
Sphingosinicella xenopeptidilytica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingosinicella xenopeptidilytica
Q52VH2
-
-
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
proteolytic modification
the gene encodes a beta-peptidyl aminopeptidase (BapA) of 402 amino acids that is synthesized as preprotein with a signal sequence of 29 amino acids. The enzyme is cleaved into two subunits (residues 30 to 278 and 279 to 402)
Sphingosinicella xenopeptidilytica
Purification (Commentary)
Commentary
Organism
-
Sphingosinicella xenopeptidilytica
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.4
-
pH 7.0, 30C
Sphingosinicella xenopeptidilytica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-homoAla-beta-homoLeu + H2O
-
708917
Sphingosinicella xenopeptidilytica
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
-
708917
Sphingosinicella xenopeptidilytica 3-2W4
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
-
708917
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
-
708917
Sphingosinicella xenopeptidilytica 3-2W4
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 12000 + x * 27000, SDS-PAGE
Sphingosinicella xenopeptidilytica
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Sphingosinicella xenopeptidilytica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Sphingosinicella xenopeptidilytica
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
Sphingosinicella xenopeptidilytica
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibitory effects are observed when the enzyme assay is performed in the presence of 1 mM of EDTA, CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2, CuCl2, beta-mercaptoethanol, 1,4-dithio-DL-threitol, or phenylmethylsulfonyl fluoride
Sphingosinicella xenopeptidilytica
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no activating effects are observed when the enzyme assay is performed in the presence of 1 mM of CaCl2, MgCl2, ZnCl2, CoCl2, FeCl2, MnCl2 or CuCl2
Sphingosinicella xenopeptidilytica
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
12000
-
x * 12000 + x * 27000, SDS-PAGE
Sphingosinicella xenopeptidilytica
27000
-
x * 12000 + x * 27000, SDS-PAGE
Sphingosinicella xenopeptidilytica
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
proteolytic modification
the gene encodes a beta-peptidyl aminopeptidase (BapA) of 402 amino acids that is synthesized as preprotein with a signal sequence of 29 amino acids. The enzyme is cleaved into two subunits (residues 30 to 278 and 279 to 402)
Sphingosinicella xenopeptidilytica
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sphingosinicella xenopeptidilytica
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.4
-
pH 7.0, 30C
Sphingosinicella xenopeptidilytica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-homoAla-beta-homoLeu + H2O
-
708917
Sphingosinicella xenopeptidilytica
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
-
708917
Sphingosinicella xenopeptidilytica 3-2W4
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
-
708917
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
-
708917
Sphingosinicella xenopeptidilytica 3-2W4
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 12000 + x * 27000, SDS-PAGE
Sphingosinicella xenopeptidilytica
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Sphingosinicella xenopeptidilytica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Sphingosinicella xenopeptidilytica
General Information
General Information
Commentary
Organism
metabolism
Sphingosinicella xenopeptidilytica strain 3-2W4 is able to utilize the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu as sole carbon and energy sources
Sphingosinicella xenopeptidilytica
General Information (protein specific)
General Information
Commentary
Organism
metabolism
Sphingosinicella xenopeptidilytica strain 3-2W4 is able to utilize the beta-peptides beta-homoVal-beta-homoAla-beta-homoLeu and beta-homoAla-beta-homoLeu as sole carbon and energy sources
Sphingosinicella xenopeptidilytica
Other publictions for EC 3.4.11.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731203
Arima
Mutation of active site serine ...
Pseudomonas aeruginosa
Appl. Microbiol. Biotechnol.
98
1631-1640
2014
-
-
1
-
1
-
-
3
-
-
3
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
2
3
2
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
2
1
-
-
2
3
2
-
1
-
-
-
-
-
-
-
708080
Heck
Enzyme-catalyzed formation of ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
Chem. Biodivers.
4
2016-2030
2007
-
2
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708078
Heck
Enzymatic degradation of beta- ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
Chem. Biodivers.
3
1325-1348
2006
-
-
-
-
-
-
-
6
-
-
-
-
-
9
-
-
2
-
-
-
-
-
60
-
2
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
2
-
-
-
-
60
-
2
-
-
6
2
-
-
-
-
-
-
-
6
6
708467
Geueke
Bacterial beta-peptidyl aminop ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
FEBS J.
273
5261-5272
2006
-
-
-
-
-
-
4
6
-
-
8
-
-
9
-
1
2
-
-
-
2
-
46
1
2
-
4
6
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
6
-
-
8
-
-
-
1
2
-
-
2
-
46
1
2
-
4
6
4
-
-
-
-
-
-
-
8
8
708917
Geueke
A novel beta-peptidyl aminopep ...
Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
J. Bacteriol.
187
5910-5917
2005
1
-
-
-
-
-
1
-
-
1
2
-
-
8
-
1
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
1
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-