BRENDA - Enzyme Database show
show all sequences of 3.4.11.25

Enzymatic degradation of beta- and mixed alpha,beta-oligopeptides

Heck, T.; Limbach, M.; Geueke, B.; Zacharias, M.; Gardiner, J.; Kohler, H.P.; Seebach, D.; Chem. Biodivers. 3, 1325-1348 (2006)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.76
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
1.23
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
1.45
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
4.4
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
8.1
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sphingosinicella microcystinivorans
A0MTQ2
-
-
Sphingosinicella microcystinivorans Y2
A0MTQ2
-
-
Sphingosinicella xenopeptidilytica
Q52VH2
-
-
Sphingosinicella xenopeptidilytica 3-2W4
Q52VH2
-
-
Purification (Commentary)
Commentary
Organism
-
Sphingosinicella microcystinivorans
-
Sphingosinicella xenopeptidilytica
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
completely degraded to the corresponding beta-homoamino acids within 312 h
708078
Sphingosinicella xenopeptidilytica
beta-homoAla + beta-homoLys + beta-homoPhe
-
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
completely degraded to the corresponding beta-homoamino acids within 312 h
708078
Sphingosinicella microcystinivorans
beta-homoAla + beta-homoLys + beta-homoPhe
-
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
completely degraded to the corresponding beta-homoamino acids within 312 h
708078
Sphingosinicella xenopeptidilytica 3-2W4
beta-homoAla + beta-homoLys + beta-homoPhe
-
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
completely degraded to the corresponding beta-homoamino acids within 312 h
708078
Sphingosinicella microcystinivorans Y2
beta-homoAla + beta-homoLys + beta-homoPhe
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans Y2
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans Y2
beta-Ala + L-His
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708078
Sphingosinicella xenopeptidilytica
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708078
Sphingosinicella microcystinivorans
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708078
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708078
Sphingosinicella microcystinivorans Y2
beta-Ala + p-nitroaniline
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
preferred substrate
708078
Sphingosinicella microcystinivorans
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
hydrolysis at 31% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-p-nitroanilide + H2O
-
708078
Sphingosinicella xenopeptidilytica
beta-homoAla + p-nitroaniline
-
-
-
?
beta-homoAla-p-nitroanilide + H2O
-
708078
Sphingosinicella microcystinivorans
beta-homoAla + p-nitroaniline
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoArg + Ile-beta-homoTyr
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoArg + Ile-beta-homoTyr
-
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoGln + Ile-beta-homoTyr
-
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
hydrolysis at 0.25% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoGln + Ile-beta-homoTyr
-
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoHis-Ile-beta-homoTyr
-
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoHis-Ile-beta-homoTyr
-
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoLeu + Ile-beta-homoTyr
-
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
hydrolysis at 54% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoLeu + Ile-beta-homoTyr
-
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
hydrolysis at 0.48% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoLys + Ile-beta-homoTyr
-
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
hydrolysis at 0.5% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoLys + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoPhe + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
hydrolysis at 19% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoPhe + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-p-nitroanilide + H2O
-
708078
Sphingosinicella xenopeptidilytica
beta-homoPhe + p-nitroaniline
-
-
-
?
beta-homoPhe-p-nitroanilide + H2O
-
708078
Sphingosinicella microcystinivorans
beta-homoPhe + p-nitroaniline
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoSer + Ile-beta-homoTyr
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
hydrolysis at 2.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoSer + Ile-beta-homoTyr
-
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
hydrolysis at 1.6% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoThr + Ile-beta-homoTyr
-
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
hydrolysis at 1.9% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoThr + Ile-beta-homoTyr
-
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
hydrolysis at 1.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoTrp + Ile-beta-homoTyr
-
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
hydrolysis at 1.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoTrp + Ile-beta-homoTyr
-
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
hydrolysis at 13% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoTyr + Ile-beta-homoTyr
-
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
hydrolysis at 7% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoTyr + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
hydrolysis at 27% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
hydrolysis at 89% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
hydrolysis at 0.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoArg-beta-homoArg
-
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
hydrolysis at 1.4% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoArg-beta-homoArg
-
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
completely hydrolyzed. Hydrolysis at 15% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoIle + beta-homoTyr
-
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoIle + beta-homoTyr
-
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
completely hydrolyzed. Hydrolysis at 20% compared to hydrolysis of beta-(3-homoVal)-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoIle + Tyr
-
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoIle + Tyr
-
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
only the N-terminal beta-homoVal is released. Hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
only the N-terminal beta-homoVal is released. Hydrolysis at 28% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-Ile-Tyr + H2O
only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + Ile-Tyr
-
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
hydrolysis at 0.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
D-beta-homoVal + Ile-beta-homoTyr
-
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
hydrolysis at 0.8% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
D-beta-homoVal + Ile-beta-homoTyr
-
-
-
?
additional information
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
708078
Sphingosinicella xenopeptidilytica
?
-
-
-
-
additional information
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
708078
Sphingosinicella microcystinivorans
?
-
-
-
-
additional information
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
708078
Sphingosinicella xenopeptidilytica 3-2W4
?
-
-
-
-
additional information
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
708078
Sphingosinicella microcystinivorans Y2
?
-
-
-
-
Val-Ile-Tyr + H2O
only the N-terminal beta-homoVal is released. Preferred substrate
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + Ile-Tyr
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Sphingosinicella microcystinivorans
25
-
assay at
Sphingosinicella xenopeptidilytica
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.13
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.22
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.75
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
2.89
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
13.2
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
30.2
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Sphingosinicella microcystinivorans
8
-
assay at
Sphingosinicella xenopeptidilytica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.7
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.76
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
1.23
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
1.45
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
4.4
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
8.1
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
Purification (Commentary) (protein specific)
Commentary
Organism
-
Sphingosinicella microcystinivorans
-
Sphingosinicella xenopeptidilytica
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
completely degraded to the corresponding beta-homoamino acids within 312 h
708078
Sphingosinicella xenopeptidilytica
beta-homoAla + beta-homoLys + beta-homoPhe
-
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
completely degraded to the corresponding beta-homoamino acids within 312 h
708078
Sphingosinicella microcystinivorans
beta-homoAla + beta-homoLys + beta-homoPhe
-
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
completely degraded to the corresponding beta-homoamino acids within 312 h
708078
Sphingosinicella xenopeptidilytica 3-2W4
beta-homoAla + beta-homoLys + beta-homoPhe
-
-
-
?
(beta-homoAla-beta-homoLys-beta-homoPhe)2 + H2O
completely degraded to the corresponding beta-homoamino acids within 312 h
708078
Sphingosinicella microcystinivorans Y2
beta-homoAla + beta-homoLys + beta-homoPhe
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
hydrolysis at 0.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-Ile-beta-homoTyr + H2O
hydrolysis at 1.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans Y2
beta-Ala + Ile-beta-homoTyr
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine. Hydrolysis at 0.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + L-His
-
-
-
?
beta-Ala-L-His + H2O
i.e. carnosine. Hydrolysis at 2% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans Y2
beta-Ala + L-His
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708078
Sphingosinicella xenopeptidilytica
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708078
Sphingosinicella microcystinivorans
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708078
Sphingosinicella xenopeptidilytica 3-2W4
beta-Ala + p-nitroaniline
-
-
-
?
beta-Ala-p-nitroanilide + H2O
-
708078
Sphingosinicella microcystinivorans Y2
beta-Ala + p-nitroaniline
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
preferred substrate
708078
Sphingosinicella microcystinivorans
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-beta-homoLeu + H2O
hydrolysis at 31% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoAla + beta-homoLeu
-
-
-
?
beta-homoAla-p-nitroanilide + H2O
-
708078
Sphingosinicella xenopeptidilytica
beta-homoAla + p-nitroaniline
-
-
-
?
beta-homoAla-p-nitroanilide + H2O
-
708078
Sphingosinicella microcystinivorans
beta-homoAla + p-nitroaniline
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoArg + Ile-beta-homoTyr
-
-
-
?
beta-homoArg-Ile-beta-homoTyr + H2O
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoArg + Ile-beta-homoTyr
-
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoGln + Ile-beta-homoTyr
-
-
-
?
beta-homoGln-Ile-beta-homoTyr + H2O
hydrolysis at 0.25% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoGln + Ile-beta-homoTyr
-
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
hydrolysis at 0.2% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoHis-Ile-beta-homoTyr
-
-
-
?
beta-homoHis-Ile-beta-homoTyr + H2O
hydrolysis at 0.35% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoHis-Ile-beta-homoTyr
-
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoLeu + Ile-beta-homoTyr
-
-
-
?
beta-homoLeu-Ile-beta-homoTyr + H2O
hydrolysis at 54% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoLeu + Ile-beta-homoTyr
-
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
hydrolysis at 0.48% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoLys + Ile-beta-homoTyr
-
-
-
?
beta-homoLys-Ile-beta-homoTyr + H2O
hydrolysis at 0.5% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoLys + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoPhe + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-Ile-beta-homoTyr + H2O
hydrolysis at 19% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoPhe + Ile-beta-homoTyr
-
-
-
?
beta-homoPhe-p-nitroanilide + H2O
-
708078
Sphingosinicella xenopeptidilytica
beta-homoPhe + p-nitroaniline
-
-
-
?
beta-homoPhe-p-nitroanilide + H2O
-
708078
Sphingosinicella microcystinivorans
beta-homoPhe + p-nitroaniline
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
hydrolysis at 12% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoSer + Ile-beta-homoTyr
-
-
-
?
beta-homoSer-Ile-beta-homoTyr + H2O
hydrolysis at 2.7% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoSer + Ile-beta-homoTyr
-
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
hydrolysis at 1.6% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoThr + Ile-beta-homoTyr
-
-
-
?
beta-homoThr-Ile-beta-homoTyr + H2O
hydrolysis at 1.9% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoThr + Ile-beta-homoTyr
-
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
hydrolysis at 1.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoTrp + Ile-beta-homoTyr
-
-
-
?
beta-homoTrp-Ile-beta-homoTyr + H2O
hydrolysis at 1.3% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoTrp + Ile-beta-homoTyr
-
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
hydrolysis at 13% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoTyr + Ile-beta-homoTyr
-
-
-
?
beta-homoTyr-Ile-beta-homoTyr + H2O
hydrolysis at 7% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoTyr + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
hydrolysis at 27% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoAla-beta-homoLeu + H2O
hydrolysis at 89% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoAla-beta-homoLeu
-
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
hydrolysis at 0.2% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoArg-beta-homoArg
-
-
-
?
beta-homoVal-beta-homoArg-beta-homoArg + H2O
hydrolysis at 1.4% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoArg-beta-homoArg
-
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
completely hydrolyzed. Hydrolysis at 15% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoIle + beta-homoTyr
-
-
-
?
beta-homoVal-beta-homoIle-beta-homoTyr + H2O
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoIle + beta-homoTyr
-
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
completely hydrolyzed. Hydrolysis at 20% compared to hydrolysis of beta-(3-homoVal)-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + beta-homoIle + Tyr
-
-
-
?
beta-homoVal-beta-homoIle-Tyr + H2O
completely hydrolyzed. Hydrolysis at 5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + beta-homoIle + Tyr
-
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
only the N-terminal beta-homoVal is released. Hydrolysis at 15% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-Ile-beta-homoTyr + H2O
only the N-terminal beta-homoVal is released. Hydrolysis at 28% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + Ile-beta-homoTyr
-
-
-
?
beta-homoVal-Ile-Tyr + H2O
only the N-terminal beta-homoVal is released. Hydrolysis at 32% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
beta-homoVal + Ile-Tyr
-
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
hydrolysis at 0.5% compared to hydrolysis of beta-homoAla-beta-homoLeu
708078
Sphingosinicella microcystinivorans
D-beta-homoVal + Ile-beta-homoTyr
-
-
-
?
D-beta-homoVal-Ile-beta-homoTyr + H2O
hydrolysis at 0.8% compared to hydrolysis of beta-homoVal-Ile-Tyr
708078
Sphingosinicella xenopeptidilytica
D-beta-homoVal + Ile-beta-homoTyr
-
-
-
?
additional information
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
708078
Sphingosinicella xenopeptidilytica
?
-
-
-
-
additional information
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
708078
Sphingosinicella microcystinivorans
?
-
-
-
-
additional information
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
708078
Sphingosinicella xenopeptidilytica 3-2W4
?
-
-
-
-
additional information
all peptides carrying a beta-homoamino acid at the N-terminus are hydrolyzed. Tripeptides with an N-terminal alpha-amino acid are not degraded
708078
Sphingosinicella microcystinivorans Y2
?
-
-
-
-
Val-Ile-Tyr + H2O
only the N-terminal beta-homoVal is released. Preferred substrate
708078
Sphingosinicella xenopeptidilytica
beta-homoVal + Ile-Tyr
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Sphingosinicella microcystinivorans
25
-
assay at
Sphingosinicella xenopeptidilytica
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.13
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.22
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.75
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
2.89
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
13.2
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
30.2
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Sphingosinicella microcystinivorans
8
-
assay at
Sphingosinicella xenopeptidilytica
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.029
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.092
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.154
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
3.8
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
18.7
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
24.6
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.029
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
0.092
-
beta-Ala-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
0.154
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
3.8
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella microcystinivorans
18.7
-
beta-homoAla-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
24.6
-
beta-homoPhe-p-nitroanilide
pH 8.0, 25°C
Sphingosinicella xenopeptidilytica
Other publictions for EC 3.4.11.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731203
Arima
Mutation of active site serine ...
Pseudomonas aeruginosa
Appl. Microbiol. Biotechnol.
98
1631-1640
2014
-
-
1
-
1
-
-
3
-
-
3
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
2
3
2
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
2
1
-
-
2
3
2
-
1
-
-
-
-
-
-
-
708080
Heck
Enzyme-catalyzed formation of ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
Chem. Biodivers.
4
2016-2030
2007
-
2
-
-
-
-
-
-
-
-
-
-
-
13
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
708078
Heck
Enzymatic degradation of beta- ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
Chem. Biodivers.
3
1325-1348
2006
-
-
-
-
-
-
-
6
-
-
-
-
-
9
-
-
2
-
-
-
-
-
60
-
2
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
2
-
-
-
-
60
-
2
-
-
6
2
-
-
-
-
-
-
-
6
6
708467
Geueke
Bacterial beta-peptidyl aminop ...
Sphingosinicella microcystinivorans, Sphingosinicella microcystinivorans Y2, Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
FEBS J.
273
5261-5272
2006
-
-
-
-
-
-
4
6
-
-
8
-
-
9
-
1
2
-
-
-
2
-
46
1
2
-
4
6
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
6
-
-
8
-
-
-
1
2
-
-
2
-
46
1
2
-
4
6
4
-
-
-
-
-
-
-
8
8
708917
Geueke
A novel beta-peptidyl aminopep ...
Sphingosinicella xenopeptidilytica, Sphingosinicella xenopeptidilytica 3-2W4
J. Bacteriol.
187
5910-5917
2005
1
-
-
-
-
-
1
-
-
1
2
-
-
8
-
1
1
-
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
1
2
-
-
-
1
1
-
-
1
-
4
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-