Inhibitors | Comment | Organism | Structure |
---|---|---|---|
fluoride | - |
Streptomyces griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00229 | - |
Leu-4-nitroanilide | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.00242 | - |
Leu-4-nitroanilide | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.093 | - |
Leu-4-nitroanilide | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.15 | - |
Leu-4-nitroanilide | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.19 | - |
Leu-4-nitroanilide | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.213 | - |
Leu-4-nitroanilide | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.88 | - |
Leu-4-nitroanilide | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
3.27 | - |
Leu-4-nitroanilide | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
3.31 | - |
Leu-4-nitroanilide | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
3.8 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
3.9 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
4.5 | - |
bis(4-nitrophenyl) phosphate | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
9.5 | - |
bis(4-nitrophenyl) phosphate | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
9.7 | - |
bis(4-nitrophenyl) phosphate | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
10.6 | - |
bis(4-nitrophenyl) phosphate | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
11 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
12.3 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
12.8 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE Sephacel column | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bis(4-nitrophenyl) phosphate + H2O | assay at pH 8.0, 30°C | Streptomyces griseus | ? | - |
? | |
Leu-4-nitroanilide + H2O | assay at pH 8.0, 30°C | Streptomyces griseus | Leu + 4-nitroaniline | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dinuclear aminopeptidase | - |
Streptomyces griseus |
SGAP | - |
Streptomyces griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces griseus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.01 | - |
bis(4-nitrophenyl) phosphate | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.016 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.043 | - |
bis(4-nitrophenyl) phosphate | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.0433 | - |
Leu-4-nitroanilide | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.0505 | - |
Leu-4-nitroanilide | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.081 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.087 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.1 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.45 | - |
bis(4-nitrophenyl) phosphate | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.74 | - |
bis(4-nitrophenyl) phosphate | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
1.5 | - |
Leu-4-nitroanilide | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
1.68 | - |
Leu-4-nitroanilide | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
27.5 | - |
Leu-4-nitroanilide | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
37.4 | - |
Leu-4-nitroanilide | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
41 | - |
Leu-4-nitroanilide | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
92.8 | - |
Leu-4-nitroanilide | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
101 | - |
Leu-4-nitroanilide | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Streptomyces griseus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
17 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
28 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
70 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
75 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
82 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
108 | - |
fluoride | substrate Leu-4-nitroanilide, in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.00094 | - |
bis(4-nitrophenyl) phosphate | in presence of NNi-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.00142 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.0044 | - |
bis(4-nitrophenyl) phosphate | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.00467 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.022 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.027 | - |
bis(4-nitrophenyl) phosphate | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
0.078 | - |
bis(4-nitrophenyl) phosphate | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus | |
0.1 | - |
bis(4-nitrophenyl) phosphate | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
7.89 | - |
Leu-4-nitroanilide | in presence of Cd-Cd-homo-dinuclear aminopeptidase | Streptomyces griseus | |
7.89 | - |
Leu-4-nitroanilide | in presence of Mn-Cd-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
18.9 | - |
Leu-4-nitroanilide | in presence of Ni-Ni-homo-dinuclear aminopeptidase | Streptomyces griseus | |
20.9 | - |
Leu-4-nitroanilide | in presence of Mn-Ni-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
28 | - |
Leu-4-nitroanilide | in presence of Mn-Zn-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
30.9 | - |
Leu-4-nitroanilide | in presence of Zn-Zn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
31.4 | - |
Leu-4-nitroanilide | in presence of Mn-Mn-homo-dinuclear aminopeptidase | Streptomyces griseus | |
249 | - |
Leu-4-nitroanilide | in presence of Mn-Co-hetero-dinuclear aminopeptidase | Streptomyces griseus | |
441 | - |
Leu-4-nitroanilide | in presence of Co-Co-homo-dinuclear aminopeptidase | Streptomyces griseus |