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Literature summary for 3.4.11.24 extracted from
- A moonlighting dizinc aminopeptidase from Streptomyces griseus: mechanisms for peptide hydrolysis and the 4 x 1010-fold acceleration of the alternative phosphodiester hydrolysis (2006), Biochemistry, 45, 13779-13793.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bis(p-nitrophenyl)phosphate | - |
Streptomyces griseus |  |
| fluoride | uncompetitive inhibitor toward peptide hydrolysis | Streptomyces griseus | |
| leucine | product inhibition occurs in peptide hydrolysis | Streptomyces griseus | |
| phosphate | noncompetitive inhibitor with peptide substrates, the enzyme-substrate-inhibitor ternary complex is inactive, but phosphate is a competitive inhibitor toward bis(p-nitrophenyl)phosphate hydrolysis, with Ki ranging from 2.31 to 315 mM at pH 5.0-9.0 | Streptomyces griseus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic analysis, effects of pH, solvent isotope effects, overview | Streptomyces griseus | |
| 0.18 | - |
Val-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 0.45 | - |
Leu-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 0.58 | - |
Met-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 1.4 | - |
Gly-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 3.4 | - |
bis(p-nitrophenyl)phosphate | pH 8.0, 30°C | Streptomyces griseus | |
| 5 | - |
Lys-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 7.8 | - |
Ala-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Streptomyces griseus | |
| Zn2+ | dizinc enzyme | Streptomyces griseus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces griseus | P80561 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Streptomyces griseus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Ala-4-nitroanilide + H2O | - |
Streptomyces griseus | Ala + 4-nitroaniline | - |
? | |
| bis(p-nitrophenyl)phosphate + H2O | - |
Streptomyces griseus | ? | - |
? | |
| Gly-4-nitroanilide + H2O | - |
Streptomyces griseus | Gly + 4-nitroaniline | - |
? | |
| Leu-4-nitroanilide + H2O | - |
Streptomyces griseus | Leu + 4-nitroaniline | - |
? | |
| Lys-4-nitroanilide + H2O | - |
Streptomyces griseus | Lys + 4-nitroaniline | - |
? | |
| Met-4-nitroanilide + H2O | - |
Streptomyces griseus | Met + 4-nitroaniline | - |
? | |
| additional information | the enzyme is active toward various peptides with different N-terminal side chains and specific toward hydrophobic ones, the enzyme also exhibits a significant activity toward the hydrolysis of the phosphodiester bis(p-nitrophenyl)phosphate, overview, active site structure involves the three auxiliary amino acid side chains of Tyr246, Glu131, and Arg202 that are involved in catalysis, modeling of substrate binding using the crystal structure of the enzyme, overview, the activity shows proton inventory and viscosity dependence, overview | Streptomyces griseus | ? | - |
? | |
| Val-4-nitroanilide + H2O | - |
Streptomyces griseus | Val + 4-nitroaniline | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dinuclear aminopeptidase | - |
Streptomyces griseus |
| dizinc aminopeptidase | - |
Streptomyces griseus |
| SGAP | - |
Streptomyces griseus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Streptomyces griseus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | - |
Streptomyces griseus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
Val-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 0.42 | - |
bis(p-nitrophenyl)phosphate | pH 8.0, 30°C | Streptomyces griseus | |
| 1.1 | - |
Gly-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 2.8 | - |
Lys-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 4.9 | - |
Ala-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 43.3 | - |
Met-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
| 657 | - |
Leu-4-nitroanilide | pH 8.0, 30°C | Streptomyces griseus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Streptomyces griseus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | bell-shaped pH-profile | Streptomyces griseus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Streptomyces griseus | |
| 2.31 | - |
bis(p-nitrophenyl)phosphate | pH 5.0, 30°C | Streptomyces griseus | |
| 10.3 | - |
leucine | pH 8.0, 30°C | Streptomyces griseus | |
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