Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, 18-25 mg/ml purified enzyme in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM CaCl2, plus an equal volume of sodium acetate buffer at pH 5.0 to 6.0, 16-20% w/v polyethylene glycol 4000, suspended over 1 ml reservoir solution of sodium acetate, pH 5.0-6.0, 16-20% PEG 4000, 3-4 weeks, X-ray diffraction structure determination at 47.2 to 1.9 A resolution and analysis | Streptomyces griseus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | excretion | Streptomyces griseus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
crystal structure determination | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | Streptomyces griseus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from commercial product | Streptomyces griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus | Streptomyces griseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | - |
Streptomyces griseus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, crystal structure determination | Streptomyces griseus |
Synonyms | Comment | Organism |
---|---|---|
M28.003 | Merops-ID | Streptomyces griseus |
SGAPase | - |
Streptomyces griseus |