Literature summary for 3.4.11.24 extracted from

Almog, O.; Greenblatt, H.M.; Spungin, A.; Ben-Meir, D.; Blumberg, S.; Shoham, G.
Crystallization and preliminary crystallographic analysis of Streptomyces griseus aminopeptidase (1993), J. Mol. Biol., 230, 342-344.

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Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method, 18-25 mg/ml purified enzyme in 10 mM Tris-HCl, pH 8.0, 20 mM NaCl, 1 mM CaCl2, plus an equal volume of sodium acetate buffer at pH 5.0 to 6.0, 16-20% w/v polyethylene glycol 4000, suspended over 1 ml reservoir solution of sodium acetate, pH 5.0-6.0, 16-20% PEG 4000, 3-4 weeks, X-ray diffraction structure determination at 47.2 to 1.9 A resolution and analysis	Streptomyces griseus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	excretion	Streptomyces griseus	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
30000	-	crystal structure determination	Streptomyces griseus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
peptide + H2O	Streptomyces griseus	-	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces griseus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
from commercial product	Streptomyces griseus

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus	Streptomyces griseus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
peptide + H2O	-	Streptomyces griseus	?	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 30000, crystal structure determination	Streptomyces griseus

Synonyms

Synonyms	Comment	Organism
M28.003	Merops-ID	Streptomyces griseus
SGAPase	-	Streptomyces griseus

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Release 2023.1 (January 2023)