Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 50% inhibition at pI 4.9 | Streptomyces griseus | |
EDTA | complete inhibition, activity cannot be completely restored by addition of 1 mM CaCl2 alone but together with 0.0001 mM ZnCl2 by 90% | Streptomyces griseus | |
EGTA | complete inhibition, activity can be completely restored by addition of 1 mM CaCl2 | Streptomyces griseus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates about 6fold, non-cooperative binding | Streptomyces griseus | |
Zn2+ | metalloprotease, dependent on | Streptomyces griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzymes are retarded on the Superose gel | Streptomyces griseus |
21000 | - |
API, gel filtration | Streptomyces griseus |
22500 | - |
APII, gel filtration | Streptomyces griseus |
33000 | - |
1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE | Streptomyces griseus |
34000 | - |
1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE | Streptomyces griseus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptide + H2O | Streptomyces griseus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces griseus | P80561 | - |
- |
Purification (Comment) | Organism |
---|---|
from commercial product, 2 peaks API and APII, endopeptidase is eliminated | Streptomyces griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | protease type XIV i.e. pronase E, a protease mixture form Streptomyces griseus | Streptomyces griseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
306 | - |
purified API | Streptomyces griseus |
317 | - |
purified APII | Streptomyces griseus |
461 | - |
purified API in presence of 1 mM CaCl2 | Streptomyces griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine-4-nitroanilide + H2O | - |
Streptomyces griseus | L-leucine + 4-nitroaniline | - |
? | |
peptide + H2O | - |
Streptomyces griseus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, API, SDS-PAGE, 1 * 34000, APII, SDS-PAGE | Streptomyces griseus |
Synonyms | Comment | Organism |
---|---|---|
AP | - |
Streptomyces griseus |
M28.003 | Merops-ID | Streptomyces griseus |
SGAP | - |
Streptomyces griseus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces griseus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
69 | - |
stable during heating for 5 h at 69°C as part of the purification process | Streptomyces griseus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces griseus |